ArticleProceedings. Biological sciences2019
Evidence for non-colligative function of small cryoprotectants in a freeze-tolerant insect.
Article in Proceedings. Biological sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 82 citations in OpenAlex.
- Whole testicular organ and seminal cryopreservation in the malarial mosquito Anopheles gambiae (Diptera: Culicidae).Journal of medical entomology · 2026Article
- Physiological reprogramming during cold acclimation in the predatory mite Neoseiulus bicaudus.Experimental & applied acarology · 2025Article
- Trehalose in cryopreservation. Applications, mechanisms and intracellular delivery opportunities.RSC medicinal chemistry · 2024Review
- Multi-omics Investigation of Freeze Tolerance in the Amur Sleeper, an Aquatic Ectothermic Vertebrate.Molecular biology and evolution · 2023Article
- Proteostasis in ice: the role of heat shock proteins and ubiquitin in the freeze tolerance of the intertidal mussel, Mytilus trossulus.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2023Article
- Dehydration Dynamics in Terrestrial Arthropods: From Water Sensing to Trophic Interactions.Annual review of entomology · 2023Review
- Adaptation and ecological speciation in seasonally varying environments at high latitudes:Fly · 2022Review
- Conservation Genomic Analysis of the Asian Honeybee in China Reveals Climate Factors Underlying Its Population Decline.Insects · 2022Article
- Stabilization of insect cell membranes and soluble enzymes by accumulated cryoprotectants during freezing stress.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Insect cross-tolerance to freezing and drought stress: role of metabolic rearrangement.Proceedings. Biological sciences · 2022Article
- Exploring Cold Hardiness within a Butterfly Clade: Supercooling Ability and Polyol Profiles in European Satyrinae.Insects · 2022Article
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- Coping with global warming: Adult thermal thresholds in four pestiferousFrontiers in physiology · 2022Article
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- Article
- Overwintering Physiology and Cold Tolerance of the Sunn Pest,Frontiers in physiology · 2020Article
- Changes in Energy Reserves and Gene Expression Elicited by Freezing and Supercooling in the Antarctic Midge,Insects · 2019Article
- Transcriptional analysis of insect extreme freeze tolerance.Proceedings. Biological sciences · 2019Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Freeze tolerance, the ability to survive internal ice formation, facilitates survival of some insects in cold habitats. Low-molecular-weight cryoprotectants such as sugars, polyols and amino acids are hypothesized to facilitate freeze tolerance, but their in vivo function is poorly understood. Here, we use a combination of metabolomics and manipulative experiments in vivo and ex vivo to examine the function of multiple cryoprotectants in the spring field cricket Gryllus veletis. Cold-acclimated G. veletis are freeze-tolerant and accumulate myo-inositol, proline and trehalose in their haemolymph and fat body. Injecting freeze-tolerant crickets with proline and trehalose increases survival of freezing to lower temperatures or for longer times. Similarly, exogenous myo-inositol and trehalose increase ex vivo freezing survival of fat body cells from freeze-tolerant crickets. No cryoprotectant (alone or in combination) is sufficient to confer freeze tolerance on non-acclimated, freeze-intolerant G. veletis. Given that each cryoprotectant differentially impacts survival in the frozen state, we conclude that small cryoprotectants are not interchangeable and likely function non-colligatively in insect freeze tolerance. Our study is the first to experimentally demonstrate the importance of non-colligative cryoprotectant function for insect freeze tolerance both in vivo and ex vivo, with implications for choosing new molecules for cryopreservation.
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