ArticleInternational journal for parasitology. Drugs and drug resistance2019
High-resolution melt (HRM) analysis for detection of SNPs associated with pyrethroid resistance in the southern cattle fever tick, Rhipicephalus (Boophilus) microplus (Acari: Ixodidae).
Article in International journal for parasitology. Drugs and drug resistance, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 52 citations in OpenAlex.
- Green Synthesis, Molecular Docking, and QSAR Study of Coumarin Derivatives as Acaricidal Agents AgainstMolecules (Basel, Switzerland) · 2026Article
- Genotypic detection of synthetic pyrethroid and organophosphate resistance-associated mutations in Rhipicephalus (Boophilus) microplus infesting ruminants across three agro-ecological zones in Pakistan.Experimental & applied acarology · 2026Article
- Chemical Composition and Ixodicidal Activity ofACS omega · 2026Article
- Species Diversity and Molecular Screening of Pyrethroid Resistance Mediated by the Voltage-Gated Sodium Channel in Ixodid Ticks from Puducherry, India.Pathogens (Basel, Switzerland) · 2026Article
- Snapback high resolution melting analysis for the rapid detection and differentiation of the L925 mutations associated with resistance to synthetic pyrethroids in Varroa destructor.Experimental & applied acarology · 2026Article
- Acaricidal activity of small molecule antagonists of the tick kinin receptor against Rhipicephalus microplus acaricide-susceptible and resistant strains.Pest management science · 2026Article
- A rapid tick exposure test for monitoring acaricide resistance in Rhipicephalus sanguineus sensu lato ticks on dogs.Parasites & vectors · 2024Article
- Current profile of phenotypic pyrethroid resistance in Rhipicephalus microplus (Acari: Ixodidae) populations sampled from Marathwada region of Maharashtra state, India.Parasitology research · 2024Article
- Molecular assessment of voltage-gated sodium channel (VGSC) gene mutations in Rhipicephalus microplus from Guangxi, China.Parasites & vectors · 2024Article
- Genetic Diversity ofPathogens (Basel, Switzerland) · 2024Review
- Chemical Composition and Acaricidal Activity of Essential Oil of Lavandula dentata L. on Engorged Females of Rhipicephalus (Boophilus) microplus (Acari: Ixodidae).Acta parasitologica · 2024Article
- Application of the TaqMan ARMS-PCR Approach for Genotyping Drug-Induced Hearing Loss Using Dried Blood Samples.Current issues in molecular biology · 2024Article
- Genomic assessment of targets implicated in Rhipicephalus microplus acaricide resistance.PloS one · 2024Article
- Efficacy of the Vaccine Candidate Based on the P0 Peptide againstPathogens (Basel, Switzerland) · 2023Article
- Assessment of cypermethrin and amitraz resistance and molecular profiling of voltage-gated sodium channel and octopamine tyramine genes ofFrontiers in cellular and infection microbiology · 2023Article
- A review of physiological resistance to insecticide stress in3 Biotech · 2022Review
- Acaricides Resistance in Ticks: Selection, Diagnosis, Mechanisms, and Mitigation.Frontiers in cellular and infection microbiology · 2022Review
- Deltamethrin resistant alleles predominate in Rhipicephalus sanguineus sensu lato in South India.Experimental & applied acarology · 2021Article
- Effect of polyphenols extracted fromJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2021Article
- Differentiation of Mitragyna speciosa, a narcotic plant, from allied Mitragyna species using DNA barcoding-high-resolution melting (Bar-HRM) analysis.Scientific reports · 2021Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The southern cattle fever tick, Rhipicephalus (Boophilus) microplus, is the most economically important ectoparasite of cattle worldwide. A limitation for sustainable control and eradication is the emergence of acaricide resistance among tick populations. Molecular diagnostic tools offer the opportunity to detect resistance rapidly, which can be complemented with confirmatory bioassays with larvae and adult ticks that are more resource and time consuming to generate. Synthetic pyrethroid resistance is one of the most prevalent and well-studied forms of resistance in arthropods, being linked with target site alterations in the sodium ion channel gene. Here, we report research on a novel molecular method to detect mutations in the para-sodium channel gene of R. microplus associated with acaricide resistance that is based on quantitative PCR high-resolution melt (HRM) analysis. Genomic DNA fragments of domains II and III of the para-sodium channel gene were amplified by real-time PCR in the presence of EVA
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