ArticlePloS one2019
Transcriptional organization, regulation and functional analysis of flhF and fleN in Pseudomonas putida.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 31 citations in OpenAlex.
- Transcriptome Analysis Reveals the Molecular Mechanism ofFoods (Basel, Switzerland) · 2025Article
- Article
- Flip the switch: the role of FleQ in modulating the transition between the free-living and sessile mode of growth inJournal of bacteriology · 2024Review
- Adaption ofMicroorganisms · 2023Review
- FleQ, FleN and c-di-GMP coordinately regulate cellulose production inFrontiers in molecular biosciences · 2023Article
- Boolean model of the gene regulatory network ofFrontiers in microbiology · 2023Article
- Tryptone in Growth Media EnhancesMicroorganisms · 2022Article
- High-throughput identification of genes influencing the competitive ability to obtain nutrients and performance of biocontrol in Pseudomonas putida JBC17.Scientific reports · 2022Article
- Spoilage of tilapia by Pseudomonas putida with different adhesion abilities.Current research in food science · 2022Article
- Role of the major determinant of polar flagellation FlhG in the endoflagella-containing spirochete Leptospira.Molecular microbiology · 2021Article
- Review
- Interdependent Polar Localization of FlhF and FlhG and Their Importance for Flagellum Formation ofFrontiers in microbiology · 2021Article
- Phenotypic and genomic characterization ofCurrent research in food science · 2021Article
- FlhF regulates the number and configuration of periplasmic flagella in Borrelia burgdorferi.Molecular microbiology · 2020Article
- Killing Two Birds With One Stone - Strain Engineering Facilitates the Development of a Unique Rhamnolipid Production Process.Frontiers in bioengineering and biotechnology · 2020Article
- GTP-Dependent FlhF Homodimer Supports Secretion of a Hemolysin inFrontiers in microbiology · 2020Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Pseudomonas putida flhA-flhF-fleN-fliA cluster encodes a component of the flagellar export gate and three regulatory elements potentially involved in flagellar biogenesis and other functions. Here we show that these four genes form an operon, whose transcription is driven from the upstream PflhA promoter. A second promoter, PflhF, provides additional transcription of the three distal genes. PflhA and PflhF are σN-dependent, activated by the flagellar regulator FleQ, and negatively regulated by FleN. Motility, surface adhesion and colonization defects of a transposon insertion mutant in flhF revealed transcriptional polarity on fleN and fliA, as the former was required for strong surface adhesion and biofilm formation, and the latter was required for flagellar synthesis. On the other hand, FlhF and FleN were necessary to attain proper flagellar location and number for a fully functional flagellar complement. FleN, along with FleQ and the second messenger c-di-GMP differentially regulated transcription of lapA and the bcs operon, encoding a large adhesion protein and cellulose synthase. FleQ positively regulated the PlapA promoter and activation was antagonized by FleN and c-di-GMP. PbcsD was negatively regulated by FleQ and FleN, and repression was antagonized by c-di-GMP. FleN promoted FleQ binding to both PlapA and PbcsD in vitro, while c-di-GMP antagonized interaction with PbcsD and stimulated interaction with PlapA. A single FleQ binding site in PlapA was critical to activation in vivo. Our results suggest that FleQ, FleN and c-di-GMP cooperate to coordinate the regulation of flagellar motility and biofilm development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.