ArticleNeuroscience bulletin2019
Rapid and Sparse Labeling of Neurons Based on the Mutant Virus-Like Particle of Semliki Forest Virus.
Article in Neuroscience bulletin, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 12 citations in OpenAlex.
- Dual adeno-associated virus system for selective and sparse labeling of astrocytes.Neural regeneration research · 2026Article
- Virus-Based Neural Circuit Tracing.Advances in neurobiology · 2024Review
- O-GlcNAcylation Is Required for the Survival of Cerebellar Purkinje Cells by Inhibiting ROS Generation.Antioxidants (Basel, Switzerland) · 2023Article
- Review
- Viral Tools for Neural Circuit Tracing.Neuroscience bulletin · 2022Review
- Sequencing-Based High-Throughput Neuroanatomy: From Mapseq to Bricseq and Beyond.Neuroscience bulletin · 2021Article
- Neuronal Network Dissection with Neurotropic Virus Tracing.Neuroscience bulletin · 2020Article
- Scalable volumetric imaging for ultrahigh-speed brain mapping at synaptic resolution.National science review · 2019Article
- Optimization of the Fluorescent Protein Expression Level Based on Pseudorabies Virus Bartha Strain for Neural Circuit Tracing.Frontiers in neuroanatomy · 2019Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sparse labeling of neurons contributes to uncovering their morphology, and rapid expression of a fluorescent protein reduces the experiment range. To achieve the goal of rapid and sparse labeling of neurons in vivo, we established a rapid method for depicting the fine structure of neurons at 24 h post-infection based on a mutant virus-like particle of Semliki Forest virus. Approximately 0.014 fluorescent focus-forming units of the mutant virus-like particle transferred enhanced green fluorescent protein into neurons in vivo, and its affinity for neurons in vivo was stronger than for neurons in vitro and BHK21 (baby hamster kidney) cells. Collectively, the mutant virus-like particle provides a robust and convenient way to reveal the fine structure of neurons and is expected to be a helper virus for combining with other tools to determine their connectivity. Our work adds a new tool to the approaches for rapid and sparse labeling of neurons in vivo.
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