ArticleFish physiology and biochemistry2019
Establishment and characterization of a fibroblast-like cell line from the muscle of turbot (Scophthalmus maximus L.).
Article in Fish physiology and biochemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 36 citations in OpenAlex.
- In Vitro Fish Cell Culture: From Primary Muscle Cells to Cell-Based Meat in Cyprinidae.Biology · 2026Review
- Myogenic Potential inACS omega · 2026Article
- Role ofFood chemistry. Molecular sciences · 2025Article
- Establishment and Characterization of Novel Channa striata Muscle Cell Line for Scaffold-Based Micro-tissue Production.Marine biotechnology (New York, N.Y.) · 2025Article
- Establishment and characterization of muscle cell line of spotted seabass (Lateolabrax maculatus).Fish physiology and biochemistry · 2025Article
- Establishment and Characterization of Hepatocyte Line from Turbot (Scophthalmus maximus L.) and Its Application in the Study of Glucose Metabolism.Marine biotechnology (New York, N.Y.) · 2025Article
- In vitro protein expression profile of cultivated muscle cells from Labeo rohita.Scientific reports · 2025Article
- Establishment, characterization, and sensory characteristics (taste and flavor) of an immortalized muscle cell line from the seven-band grouper Epinephelus septemfasciatus: implications for cultured seafood applications.In vitro cellular & developmental biology. Animal · 2025Article
- Establishment and Characterization of Continuous Satellite Muscle Cells from Olive Flounder (Cells · 2023Article
- Development of a cell line from skeletal trunk muscle of the fishCytotechnology · 2023Article
- Functional characterization of Labeo rohita muscle cell line for in vitro research.Molecular biology reports · 2023Article
- Derivation and characterization of new cell line from intestine of turbot (Scophthalmus maximus).In vitro cellular & developmental biology. Animal · 2023Article
- Distinguishing between ante factum and post factum properties of animal cell lines and demonstrating their use in grouping ray-finned fish cell lines into invitromes.In vitro cellular & developmental biology. Animal · 2023Review
- An established kidney cell line from humpback grouper (Cromileptes altivelis) and its susceptibility to bacteria and heavy metals.Fish physiology and biochemistry · 2022Article
- Cellular Aquaculture: Prospects and Challenges.Micromachines · 2022Review
- Establishment and characterization of a new cell line from the muscle of humpback grouper (Cromileptes altivelis).Fish physiology and biochemistry · 2020Article
- Developments in marine invertebrate primary culture reveal novel cell morphologies in the model bivalvePeerJ · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
A continuous fibroblast-like cell line, TMF (turbot muscle fibroblasts), was established from juvenile turbot Scophthalmus maximus muscle with the method of trypsin digestion. It has been subcultured more than 60 passages for over 150 days. The TMF cells were cultured in L-15 medium supplemented with HEPES, fetal bovine serum (FBS), GlutaMAX, and basic fibroblast growth factor (bFGF). The optimal temperature for TMF culture was 24 °C. TMF cells were predominantly composed of fibroblastic-like cells, and the transcription factor 4 (TCF-4) was highly expressed in TMF cells. Chromosome analysis revealed that it had a diploid chromosome number of 2n = 44. The transfection efficiency achieved 54.95 ± 6.59%, and the cell mortality rate was about 8.70% when transfected with the nucleofection method. Meanwhile, the TMF cells showed a sensitive response to amino acid levels and activation target of rapamycin (TOR) signaling pathway. These results indicate that TMF was a potential tool to explore the signal transduction of teleost in vitro.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.