Evidence map›Paper›PMID 30888514›Full record

ArticleClinical oral investigations2019

Is it really penetration? Part 2. Locomotion of Enterococcus faecalis cells within dentinal tubules of bovine teeth.

Jasmin Kirsch, Sabine Basche, Jörg Neunzehn, Maria Dede, Martin Dannemann, Christian Hannig, Marie-Theres Weber

Abstract read
PubMed Publisher
In one paragraph

Article in Clinical oral investigations, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed, 2 pooled it
0.7field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 2 syntheses or guidelines pooled it, 10 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Article
  4. Review
  5. Article
  6. Efficacy of Endodontic Disinfection Protocols in anJournal of functional biomaterials · 2023
    Article
  7. Role ofJournal of oral microbiology · 2023
    Review
  8. Article
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Jasmin KirschClinic of Operative Dentistry, Medical Faculty Carl Gustav Carus, TU Dresden, Fetscherstr.74, 01307, Dresden, Germany. Jasmin.Kirsch@uniklinikum-dresden.de.ORCID http://orcid.org/0000-0002-0874-6523
Sabine BascheClinic of Operative Dentistry, Medical Faculty Carl Gustav Carus, TU Dresden, Fetscherstr.74, 01307, Dresden, Germany.
Jörg NeunzehnVertriebsgesellschaft GmbH, Geistlich Biomaterials, Schneidweg 5, 76534, Baden-Baden, Germany.
Maria DedeClinic of Operative Dentistry, Medical Faculty Carl Gustav Carus, TU Dresden, Fetscherstr.74, 01307, Dresden, Germany.
Martin DannemannInstitute of Lightweight Engineering and Polymer Technology (ILK), Faculty of Mechanical Engineering, Technische Universität Dresden, Holbeinstrasse 3, 01307, Dresden, Germany.
Christian HannigClinic of Operative Dentistry, Medical Faculty Carl Gustav Carus, TU Dresden, Fetscherstr.74, 01307, Dresden, Germany.
Marie-Theres WeberClinic of Operative Dentistry, Medical Faculty Carl Gustav Carus, TU Dresden, Fetscherstr.74, 01307, Dresden, Germany.
University Hospital Carl Gustav Carus · DETechnische Universität Dresden · DE

Funding

Deutsche Forschungsgemeinschaft DA 1701/1-1Deutsche Forschungsgemeinschaft WE 5838/1-1
6 · The paper itself

Abstract

objectiveThe aim of the present vitro study was to examine the question whether devitalized Enterococcus faecalis (E. faecalis) cells can migrate into dentinal tubules and if that process takes place in a time-dependent manner.

designSixty bovine root canals were incubated with devitalized and vital streptomycin-resistant E. faecalis strains after root canal enlargement (size 80, taper .02) with 3% NaOCl solution. Incubation times 7, 14, 21, 28, 35, and 42 days. Samples were processed for analysis by scanning electron microscopy (SEM) and DAPI (4',6-diamidino-2-phenylindole) staining. The penetration depth was calculated with the measurement tool of the Axio Vision program (Zeiss, Jena, Germany). Statistical analysis was performed by Kruskal-Wallis (α = 0.05) and Mann-Whitney U test (p < 0.05).

resultsDevitalized E. faecalis strains were able to migrate into dentinal tubules. The total number and penetration depth of devitalized E. faecalis cells was lower compared to the vital suspension of E. faecalis. It was noted, that bacterial penetration was not common to all of the dentinal tubules in the vital E. faecalis control and especially in the devitalized control. The migration took place in a time-dependent migration characteristic.

conclusionsDevitalized E. faecalis cells are still able to migrate into the dentinal tubules due to possible electrokinetic and osmotic processes. Thereby, increased exposure times lead to a time-dependent penetration characteristic. CLINICAL RELEVANCE: Since devitalized bacteria can migrate as well into dentinal tubules, the presence of bacteria within dentinal tubules cannot be interpreted as a failure of tested preparation regimens.

Indexed as

AnimalsCattleDental Pulp CavityDentinEnterococcus faecalisGermanyLocomotionRoot Canal IrrigantsSodium HypochloriteRoot Canal IrrigantsSodium HypochloriteBacterial penetrationDAPI methodDentinal tubulesDevitalized bacteriaE. faecalis cellsRoot canal

Identifiers

PMID30888514
OpenAlexW2925323394

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.