ArticleDaru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences2019
In vitro anti-proliferative activities of the sterols and fatty acids isolated from the Persian Gulf sponge; Axinella sinoxea.
Article in Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed, 21 citations in OpenAlex.
- Physicochemical Characterisation and In Vitro Bioactivity of Sumac (Rhus coriaria)-Derived Exosome-Like Nanoparticles.Chemistry & biodiversity · 2026Article
- Article
- Bioassay-Guided Purification of Cytotoxic Isoflavonoids and Sesquiterpenoids ofIranian journal of biotechnology · 2025Article
- Article
- Article
- Antifungal Properties of Bioactive Compounds Isolated fromMolecules (Basel, Switzerland) · 2024Article
- Application of bioactive glasses in various dental fields.Biomaterials research · 2022Review
- Comparative metabolomic analysis reveals shared and unique chemical interactions in sponge holobionts.Microbiome · 2022Article
- An integrated approach utilizing raman spectroscopy and chemometrics for authentication and detection of adulteration of agarwood essential oils.Frontiers in chemistry · 2022Article
- Cytotoxic furanosesquiterpenoids and steroids fromPharmaceutical biology · 2021Article
- Integrating Molecular Networking andMarine drugs · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeMarine sponges are rich sources of anticancer metabolites. Axinella sinoxea is a less studied sponge, found in the Larak Island's waters, of the Persian Gulf. In the present study, we have explored the cytotoxic properties and chemical constituents of A. sinoxea.
methodsRepeated silica gel flash column chromatography of methanol extract of the Axinella sinoxea sponge, yielded fatty acid and sterol fractions. These fractions were analyzed by GC-MS and their anti-proliferative activities were evaluated by MTT assay against three human cancer cell lines including MOLT-4, MCF-7 and HT-29 as well as NIH/3 T3 fibroblast cells. The sterol-rich fractions were pooled and purified by HPLC and its sub fractions' cytotoxic activities were evaluated by MTT assay against MOLT-4 and NIH/3 T3 cells.
resultsThe GC-MS spectral analysis of a fraction eluted with hexane: diethyl ether (90: 10), resulted in the identification of twelve fatty acids, including five linear chain saturated fatty acids; tetrdecanoic acid (1), pentadecanoic acid (3), hexadecanoic acid (5), heptadecanoic acid (7), and octadecanoic acid (10); one branched chain isoprenoid fatty acid, 4,8,12-trimethyltridecanoic acid (2); four monoenoic fatty acids; 9-hexadecenoic acid (4), 7-methyl-6-hexadecanoic acid (6), 9-octadecenoic acid (8) and 11-octadecenoic acid (9) and two polyunsaturated fatty acids; 5,8,11,14-eicosatetraenoic acid (11) and 4,7,10,13,16,19-docosahexaenoic acid (12). Spectral analysis of a non-polar fraction eluted with hexane: diethyl ether (85: 15), resulted in the identification of eight steroids including: cholesta-5,22-dien-3β-ol (13), cholest-5-en-3β-ol (14), ergosta-5,22-dien-3β-ol (15), ergost-5-en-3β-ol (16), stigmasta-5,22-dien-3β-ol (17), γ-sitosterol (18), 33-norgorgosta-5,24(28)-dien-3β-ol (19) and stigmasta-5,24(28)-dien-3β-ol (20). Fatty acids-containing fraction was active against HT-29 cell line with IC
conclusionThe present study suggests A. sinoxea as a potential natural source of cancer chemotherapeutics. Graphical abstract Cytotxic constituents of Axinella sinoxea.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.