ArticleJournal of assisted reproduction and genetics2019
Fibroblast growth factor 2 regulates cumulus differentiation under the control of the oocyte.
Article in Journal of assisted reproduction and genetics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 34 citations in OpenAlex.
- The Critical Functions of FGF2, LIF and IGF1 in the Improvement of In Vitro Embryo Production.Biomolecules · 2026Review
- FGF receptor 2 signaling in granulosa cells is required for normal female fertility in mice.Reproduction (Cambridge, England) · 2025Article
- Proneurogenic Actions of FSH During Directed Differentiation of Neural Stem and Progenitor Cells from Ovarian Cortical Cells Towards the Dopaminergic Pathway.Biomedicines · 2025Article
- Triclosan Caused Oocyte Meiotic Arrest by Modulating Oxidative Stress, Organelle Dysfunctions, Autophagy, and Apoptosis in Pigs.Animals : an open access journal from MDPI · 2025Article
- Current status and hotspots of in vitro oocyte maturation: a bibliometric study of the past two decades.Journal of assisted reproduction and genetics · 2025Review
- Meiotic and developmental competence of growing pig oocytes derived from small antral follicles is enhanced in culture medium containing FGF2, LIF, and IGF1 (FLI medium).Journal of ovarian research · 2024Article
- All-trans retinoic acid and fibroblast growth factor-2 enhance the fertility rate and embryo development in polycystic ovary syndrome mouse model.Iranian journal of basic medical sciences · 2024Article
- Oocyte secreted factors control genes regulating FSH signaling and the maturation cascade in cumulus cells: the oocyte is not in a hurry.Journal of assisted reproduction and genetics · 2023Article
- Combination of CNP, MT and FLI during IVM Significantly Improved the Quality and Development Abilities of Bovine Oocytes and IVF-Derived Embryos.Antioxidants (Basel, Switzerland) · 2023Article
- Simple and Efficient Chemically Defined In Vitro Maturation and Embryo Culture System for Bovine Embryos.Animals : an open access journal from MDPI · 2022Article
- Glyphosate exposure deteriorates oocyte meiotic maturation via induction of organelle dysfunctions in pigs.Journal of animal science and biotechnology · 2022Article
- The Role of MAPK3/1 and AKT in the Acquisition of High Meiotic and Developmental Competence of Porcine Oocytes Cultured In Vitro in FLI Medium.International journal of molecular sciences · 2021Article
- Pluripotency and Growth Factors in Early Embryonic Development of Mammals: A Comparative Approach.Veterinary sciences · 2021Review
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
purposeWe first assessed regulation of FGF2 expression in cumulus cells by FSH and oocyte-secreted factors during in vitro maturation (IVM). Then, we tested the hypothesis that FGF2 regulates meiotic progression, cumulus expansion, and apoptosis in cumulus-oocyte complexes (COC) undergoing IVM.
methodsIn vitro maturation of bovine COC was utilized as a model to assess regulation of FGF2 expression by FSH and oocyte-secreted factors (via microsurgical removal of the oocyte), as well as effects of graded doses of FGF2 on meiotic progression, degree of cumulus expansion, dissociation of cumulus cells, and cumulus cells apoptosis. Expression of genes regulating functional endpoints altered by FGF2 treatment was assessed in cumulus cells by real-time PCR. Cultures were replicated 4-5 times and effects of treatments were tested by ANOVA.
resultsFGF2 mRNA expression was increased by FSH and oocyte-secreted factors during IVM. Addition of FGF2 to the IVM medium advanced meiosis resumption, decreased the ease with which cumulus cells were dissociated, and inhibited cumulus cells apoptosis. Decreased cumulus dissociation was accompanied by decreased expression of TNFAIP6.
conclusionsThis is the first study showing that FGF2 expression is regulated by the oocyte in cumulus cells. Moreover, we report novel effects of FGF2 on cumulus cell survival and extracellular matrix (ECM) quality during IVM that may favor acquisition of developmental competence and suggest physiological roles during the final steps of COC differentiation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.