ArticleScientific reports2019
Combined Raman and polarization sensitive holographic imaging for a multimodal label-free assessment of human sperm function.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 33 citations in OpenAlex.
- Label-Free Detection of Acrosome Reaction of Human Sperm Based on Diffraction Phase Microscopy.Chemical & biomedical imaging · 2026Article
- Quantitative holographic analysis in stallion spermatozoa following cryopreservation.Scientific reports · 2025Article
- Polarization-Sensitive Holotomography for Multidimensional Label-Free Imaging and Characterization of Lipid Droplets in Cancer Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Surface-Enhanced Raman Spectroscopy on Gold Nanoparticle for Sperm Quality Discrimination.Molecules (Basel, Switzerland) · 2025Article
- Correlative Raman Imaging: Development and Cancer Applications.Biosensors · 2024Review
- Cancer metabolic features allow discrimination of tumor from white blood cells by label-free multimodal optical imaging.Frontiers in bioengineering and biotechnology · 2023Article
- Dynamic Tomographic Phase Microscopy by Double Six-Pack Holography.ACS photonics · 2022Article
- Decoding the role of cytochrome c in metabolism of human spermatozoa by Raman imaging.Frontiers in cell and developmental biology · 2022Article
- Label-Free Evaluation of Chromatin Condensation in Human Normal Morphology Sperm Using Raman Spectroscopy.Reproductive sciences (Thousand Oaks, Calif.) · 2021Article
- Drying and temperature induced conformational changes of nucleic acids and stallion sperm chromatin in trehalose preservation formulations.Scientific reports · 2021Article
- Raman Microscopy: Progress in Research on Cancer Cell Sensing.Sensors (Basel, Switzerland) · 2020Review
- Holography applications toward medical field: An overview.The Indian journal of radiology & imagingArticle
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Raman microspectroscopy (RM) and polarization sensitive digital holographic imaging (PSDHI) are valuable analytical tools in biological and medical research, allowing the detection of both biochemical and morphological variations of the sample without labels or long sample preparation. Here, using this multi-modal approach we analyze in vitro human sperm capacitation and the acrosome reaction induced by heparin. The multimodal microscopy provides morphofunctional information that can assess the sperms ability to respond to capacitation stimuli (sperm function). More precisely, the birefringence analysis in sperm cells can be used as an indicator of its structural normality. Indeed, digital holography applied for polarization imaging allows for revelation of the polarization state of the sample, showing a total birefringence of the sperm head in non-reacted spermatozoa, and a birefringence localized in the post-acrosomal region in reacted spermatozoa. Additionally, RM allows the detection and spectroscopic characterization of protein/lipid delocalization in the plasma and acrosomal membranes that can be used as valuable Raman biomarkers of sperm function. Interestingly, these spectral variations can be correlated with different time phases of the cell capacitation response. Although further experimentation is required, the proposed multimodal approach could represent a potential label-free diagnostic tool for use in reproductive medicine and the diagnosis of infertility.
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