ArticleScientific reports2019
Rapid genetic and phenotypic changes in Pseudomonas aeruginosa clinical strains during ventilator-associated pneumonia.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 43 citations in OpenAlex.
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- Phenotypic convergence and collateral susceptibility development in Pseudomonas aeruginosa under antibiotic exposure in ICU patients.npj antimicrobials and resistance · 2026Article
- Comparative genomics ofJournal of bacteriology · 2025Article
- Intra-host genomic variation of serologically nontypeableMicrobiology spectrum · 2025Article
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- Prevalence of different virulence factors and their association with antimicrobial resistance among Pseudomonas aeruginosa clinical isolates from Egypt.BMC microbiology · 2023Article
- Anti-Microorganisms · 2023Review
- Rapid expansion and extinction of antibiotic resistance mutations during treatment of acute bacterial respiratory infections.Nature communications · 2022Article
- Allosteric differences dictate GroEL complementation of E. coli.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022Article
- Prediction of Potential Drug Targets and Vaccine Candidates Against Antibiotic-ResistantInternational journal of peptide research and therapeutics · 2022Article
- Quorum sensing systems and related virulence factors in Pseudomonas aeruginosa isolated from chicken meat and ground beef.Scientific reports · 2021Article
- Intraspecies heterogeneity in microbial interactions.Current opinion in microbiology · 2021Review
- From genotype to phenotype: adaptations ofMicrobial genomics · 2021Review
- Article
- Phenotypic and genotypic analysis of biofilm production by Pseudomonas aeruginosa isolates from infection and colonization samples.Revista da Sociedade Brasileira de Medicina Tropical · 2020Article
- Article
- Virulence Factors Of Carbapenem-ResistantInfection and drug resistance · 2019Article
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Treatment with antibiotics leads to the selection of isolates with increased resistance. We investigated if evolution towards resistance was associated with virulence changes, in the context of P. aeruginosa ventilator-associated pneumonia (VAP). Four patients were selected because they had multiple VAP episodes during short periods (12 days to 5 weeks), with emergence of resistance. We performed whole-genome sequencing of 12 P. aeruginosa from bronchoalveolar lavages or blood culture (3 isolates per patient). Production of quorum sensing-dependent virulence factors, serum resistance, cytotoxicity against A549 cells, biofilm production, and twitching motility were studied. Each patient was infected with a unique strain. For all patients, resistance development was explained by genetic events in ampD, mexR or oprD. Additional variations were detected in virulence- and/or fitness-associated genes (algB, gacA, groEL, lasR, mpl, pilE, pilM, rhlR) depending on the strain. We noticed a convergence towards quorum sensing deficiency, correlated with a decrease of pyocyanin and protease production, survival in serum, twitching motility and cytotoxicity. In one patient, changes in pilM and pilE were related to enhanced twitching. We show that the emergence of resistance in P. aeruginosa is associated with virulence modification, even in acute infections. The consequences of this short-term pathoadaptation need to be explored.
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