ArticleScientific reports2019
Near real-time enumeration of live and dead bacteria using a fibre-based spectroscopic device.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 36 citations in OpenAlex.
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- Novel delivery systems for controlled release of bacterial therapeutics.Trends in biotechnology · 2024Review
- Ironing out Persisters? Revisiting the Iron Chelation Strategy to Target Planktonic Bacterial Persisters Harboured in Carbapenem-ResistantMicroorganisms · 2024Article
- What happens to Bifidobacterium adolescentis and Bifidobacterium longum ssp. longum in an experimental environment with eukaryotic cells?BMC microbiology · 2024Article
- Abundance of Live and Dead Bacteriopsammon Inhabiting Sandy Ecosystems of Recreational Marine Beaches of the Southern Baltic Sea.Microbial ecology · 2023Article
- Biosensors, modern technology for the detection of cancer-associated bacteria.Biotechnology letters · 2022Review
- Novel fluorescence-based method for rapid quantification of live bacteria in river water and treated wastewater.Environmental science. Advances · 2022Article
- A rapid colorimetric lateral flow test strip for detection of liveFrontiers in microbiology · 2022Article
- Detection ofFrontiers in microbiology · 2022Article
- Rapid Detection ofMicroorganisms · 2021Article
- Early detection of drug-resistant Streptococcus pneumoniae and Haemophilus influenzae by quantitative flow cytometry.Scientific reports · 2021Article
- Near-Infrared Light-Triggered Bacterial Eradication Using a Nanowire Nanocomposite of Graphene Nanoribbons and Chitosan-Coated Silver Nanoparticles.Frontiers in chemistry · 2021Article
- Rapid and cost-effective evaluation of bacterial viability using fluorescence spectroscopy.Analytical and bioanalytical chemistry · 2019Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A rapid, cost-effective and easy method that allows on-site determination of the concentration of live and dead bacterial cells using a fibre-based spectroscopic device (the optrode system) is proposed and demonstrated. Identification of live and dead bacteria was achieved by using the commercially available dyes SYTO 9 and propidium iodide, and fluorescence spectra were measured by the optrode. Three spectral processing methods were evaluated for their effectiveness in predicting the original bacterial concentration in the samples: principal components regression (PCR), partial least squares regression (PLSR) and support vector regression (SVR). Without any sample pre-concentration, PCR achieved the most reliable results. It was able to quantify live bacteria from 10
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