ArticleScientific reports2019
Sizing biological cells using a microfluidic acoustic flow cytometer.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 42 citations in OpenAlex.
- High-Resolution Ultrasound Platform for Infant Meningitis Detection: An In Vitro Demonstration.Sensors (Basel, Switzerland) · 2024Article
- Real-time imaging of nanobubble ultrasound contrast agent flow, extravasation, and diffusion through an extracellular matrix using a microfluidic model.Lab on a chip · 2023Article
- What is the future of electrical impedance spectroscopy in flow cytometry?Biomicrofluidics · 2021Article
- Non-invasive acquisition of mechanical properties of cells via passive microfluidic mechanisms: A review.Biomicrofluidics · 2021Review
- Novel devices for isolation and detection of bacterial and mammalian extracellular vesicles.Mikrochimica acta · 2021Review
- An image-based flow cytometric approach to the assessment of the nucleus-to-cytoplasm ratio.PloS one · 2021Article
- The dance of the nanobubbles: detecting acoustic backscatter from sub-micron bubbles using ultra-high frequency acoustic microscopy.Nanoscale · 2020Article
- A novel abrasive water jet machining technique for rapid fabrication of three-dimensional microfluidic components.Biomicrofluidics · 2020Article
- Acoustic Microfluidics.Annual review of analytical chemistry (Palo Alto, Calif.) · 2020Review
- Determination of cell nucleus-to-cytoplasmic ratio using imaging flow cytometry and a combined ultrasound and photoacoustic technique: a comparison study.Journal of biomedical optics · 2019Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe a new technique that combines ultrasound and microfluidics to rapidly size and count cells in a high-throughput and label-free fashion. Using 3D hydrodynamic flow focusing, cells are streamed single file through an ultrasound beam where ultrasound scattering events from each individual cell are acquired. The ultrasound operates at a center frequency of 375 MHz with a wavelength of 4 μm; when the ultrasound wavelength is similar to the size of a scatterer, the power spectra of the backscattered ultrasound waves have distinct features at specific frequencies that are directly related to the cell size. Our approach determines cell sizes through a comparison of these distinct spectral features with established theoretical models. We perform an analysis of two types of cells: acute myeloid leukemia cells, where 2,390 measurements resulted in a mean size of 10.0 ± 1.7 μm, and HT29 colorectal cancer cells, where 1,955 measurements resulted in a mean size of 15.0 ± 2.3 μm. These results and histogram distributions agree very well with those measured from a Coulter Counter Multisizer 4. Our technique is the first to combine ultrasound and microfluidics to determine the cell size with the potential for multi-parameter cellular characterization using fluorescence, light scattering and quantitative photoacoustic techniques.
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Registered trials
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