Evidence map›Paper›PMID 30886164›Full record

ArticleScientific reports2019

The deubiquitinating enzyme USP25 binds tankyrase and regulates trafficking of the facilitative glucose transporter GLUT4 in adipocytes.

Jessica B A Sadler, Christopher A Lamb, Cassie R Welburn, Iain S Adamson, Dimitrios Kioumourtzoglou, Nai-Wen Chi, Gwyn W Gould, Nia J Bryant

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
0.9field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 30 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Review
  5. Review
  6. Review
  7. Article
  8. Article
  9. Article
  10. GLUT4 On the move.The Biochemical journal · 2022
    Article
  11. Review
  12. Review
  13. Emerging Roles of Ubiquitin-Specific Protease 25 in Diseases.Frontiers in cell and developmental biology · 2021
    Review
  14. Ubiquitin-specific peptidase 7 (USP7) and USP10 mediate deubiquitination of human NHE3 regulating its expression and activity.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020
    Article
  15. Review
  16. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Jessica B A SadlerHenry Wellcome Laboratory of Cell Biology, Institute of Molecular Cell and Systems Biology, College of Medical Veterinary and Life Sciences, University of Glasgow, Glasgow, G12 8QQ, UK.
Christopher A LambHenry Wellcome Laboratory of Cell Biology, Institute of Molecular Cell and Systems Biology, College of Medical Veterinary and Life Sciences, University of Glasgow, Glasgow, G12 8QQ, UK.
Cassie R WelburnHenry Wellcome Laboratory of Cell Biology, Institute of Molecular Cell and Systems Biology, College of Medical Veterinary and Life Sciences, University of Glasgow, Glasgow, G12 8QQ, UK.
Iain S AdamsonHenry Wellcome Laboratory of Cell Biology, Institute of Molecular Cell and Systems Biology, College of Medical Veterinary and Life Sciences, University of Glasgow, Glasgow, G12 8QQ, UK.
Dimitrios KioumourtzoglouDepartment of Biology, University of York, York, YO10 4HJ, UK.
Nai-Wen ChiDepartment of Medicine, University of California, San Diego, La Jolla, CA, 92093, USA.
Gwyn W GouldHenry Wellcome Laboratory of Cell Biology, Institute of Molecular Cell and Systems Biology, College of Medical Veterinary and Life Sciences, University of Glasgow, Glasgow, G12 8QQ, UK. gwyn.gould@glasgow.ac.uk.
Nia J BryantDepartment of Biology, University of York, York, YO10 4HJ, UK. nia.bryant@york.ac.uk.
University of Glasgow · GBUniversity of York · GBUniversity of California, San Diego · US

Funding

Medical Research Council MR/J000221/1Wellcome Trust
6 · The paper itself

Abstract

Key to whole body glucose homeostasis is the ability of fat and muscle cells to sequester the facilitative glucose transporter GLUT4 in an intracellular compartment from where it can be mobilized in response to insulin. We have previously demonstrated that this process requires ubiquitination of GLUT4 while numerous other studies have identified several molecules that are also required, including the insulin-responsive aminopeptidase IRAP and its binding partner, the scaffolding protein tankyrase. In addition to binding IRAP, Tankyrase has also been shown to bind the deubiquinating enzyme USP25. Here we demonstrate that USP25 and Tankyrase interact, and colocalise with GLUT4 in insulin-sensitive cells. Furthermore depletion of USP25 from adipocytes reduces cellular levels of GLUT4 and concomitantly blunts the ability of insulin to stimulate glucose transport. Collectively, these data support our model that sorting of GLUT4 into its insulin-sensitive store involves a cycle of ubiquitination and subsequent deubiquitination.

Indexed as

3T3-L1 CellsAdipocytesAnimalsCell MembraneCystinyl AminopeptidaseGene Knockdown TechniquesGlucoseGlucose Transporter Type 4InsulinMiceTankyrasesUbiquitinationUbiquitin ThiolesteraseCystinyl AminopeptidaseGlucoseGlucose Transporter Type 4Insulinleucyl-cystinyl aminopeptidaseSlc2a4 protein, mouseTankyrasesTnks protein, mouseUbiquitin ThiolesteraseUSP25 protein, mouse

Identifiers

PMID30886164
PMCPMC6423145
OpenAlexW2921454691

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.