Evidence map›Paper›PMID 30885254›Full record

ArticleStem cell research & therapy2019

A comprehensive characterisation of large-scale expanded human bone marrow and umbilical cord mesenchymal stem cells.

Claire Mennan, John Garcia, Sally Roberts, Charlotte Hulme, Karina Wright

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research & therapy, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.

0numbers the graph read from it
0cells of the map it votes in
54citing papers in PubMed
7.1field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

54 citing papers in PubMed, 79 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Article
  5. Review
  6. Review
  7. Article
  8. Article
  9. Review
  10. Article
  11. Article
  12. Review
  13. Article
  14. Review
  15. Article
  16. Review
  17. Article
  18. Review
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Claire MennanInstitute of Science and Technology in Medicine, Keele University, Keele, Staffordshire, ST4 7QB, UK.
John GarciaInstitute of Science and Technology in Medicine, Keele University, Keele, Staffordshire, ST4 7QB, UK.
Sally RobertsInstitute of Science and Technology in Medicine, Keele University, Keele, Staffordshire, ST4 7QB, UK.
Charlotte HulmeInstitute of Science and Technology in Medicine, Keele University, Keele, Staffordshire, ST4 7QB, UK.
Karina WrightInstitute of Science and Technology in Medicine, Keele University, Keele, Staffordshire, ST4 7QB, UK. karina.wright1@nhs.net.
Keele University · GBRobert Jones and Agnes Hunt Orthopaedic Hospital · GB

Funding

Arthritis Research UK 19425Arthritis Research UK 20253Arthritis Research UK 21156Medical Research Council MC_PC_16049Versus Arthritis 18480Versus Arthritis 20253Versus Arthritis 20815Versus Arthritis 21156
6 · The paper itself

Abstract

backgroundThe manufacture of mesenchymal stem/stromal cells (MSCs) for clinical use needs to be cost effective, safe and scaled up. Current methods of expansion on tissue culture plastic are labour-intensive and involve several 'open' procedures. We have used the closed Quantum® hollow fibre bioreactor to expand four cultures each of MSCs derived from bone marrow (BM) and, for the first time, umbilical cords (UCs) and assessed extensive characterisation profiles for each, compared to parallel cultures grown on tissue culture plastic.

methodsBone marrow aspirate was directly loaded into the Quantum®, and cells were harvested and characterised at passage (P) 0. Bone marrow cells were re-seeded into the Quantum®, harvested and further characterised at P1. UC-MSCs were isolated enzymatically and cultured once on tissue culture plastic, before loading cells into the Quantum®, harvesting and characterising at P1. Quantum®-derived cultures were phenotyped in terms of immunoprofile, tri-lineage differentiation, response to inflammatory stimulus and telomere length, as were parallel cultures expanded on tissue culture plastic.

resultsBone marrow cell harvests from the Quantum® were 23.1 ± 16.2 × 10

conclusionsThe results presented here demonstrate that the Quantum® can be used to expand large numbers of MSCs from bone marrow and umbilical cord tissues for next-generation large-scale manufacturing, without impacting on many of the properties that are characteristic of MSCs or potentially therapeutic. Using the Quantum®, we can obtain multiple MSC doses from a single manufacturing run to treat many patients. Together, our findings support the development of cheaper cell-based treatments.

Indexed as

Cell Culture TechniquesCell DifferentiationCell SeparationAdultBone Marrow CellsHumansInfant, NewbornMaleMesenchymal Stem CellsUmbilical CordBone marrowCharacterisationFlow cytometryHollow fibre bioreactorLarge-scale expansionMesenchymal stem cellsMultipotential differentiationTelomere lengthUmbilical cord

Identifiers

PMID30885254
PMCPMC6421680
OpenAlexW2941573530

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.