ArticleBMC ophthalmology2019
Cyclic stretch induced oxidative stress by mitochondrial and NADPH oxidase in retinal pigment epithelial cells.
Article in BMC ophthalmology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 29 citations in OpenAlex.
- Excessive Stretching Drives RPE Inflammation and ECM Remodeling in Ectopia Lentis Retinopathy.International journal of molecular sciences · 2026Article
- Associations between mechanical stress and epigenetic modifications in outer retinal layer remodeling in high myopia.Frontiers in medicine · 2026Review
- Lens epithelial cells senescence in cataract pathogenesis and emerging therapeutic opportunities.Frontiers in cell and developmental biology · 2026Review
- Applying low levels of strain to model nascent phenomenon of retinal pathologies.Lab on a chip · 2024Article
- Endogenous and Exogenous Regulation of Redox Homeostasis in Retinal Pigment Epithelium Cells: An Updated Antioxidant Perspective.International journal of molecular sciences · 2023Review
- Static and Dynamic: Evolving Biomaterial Mechanical Properties to Control Cellular Mechanotransduction.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Review
- The effect of vitreomacular interface in neovascular age-related macular degeneration treated with intravitreal injection of anti-VEGF.BMC ophthalmology · 2022Article
- Biosynthesis of Gold Nanoparticles by Vascular CellsFrontiers in microbiology · 2022Article
- FRET Visualization of Cyclic Stretch-Activated ERKFrontiers in cell and developmental biology · 2022Article
- Single-cell transcriptomic profiling reveals distinct mechanical responses between normal and diseased tendon progenitor cells.Cell reports. Medicine · 2021Article
- Sodium Glucose Co-Transporter 2 Inhibitors Ameliorate Endothelium Barrier Dysfunction Induced by Cyclic Stretch through Inhibition of Reactive Oxygen Species.International journal of molecular sciences · 2021Article
- Acute mechanical stress in primary porcine RPE cells induces angiogenic factor expression and in vitro angiogenesis.Journal of biological engineering · 2020Article
- Observational
- Reactive Oxygen Species (ROS), Intimal Thickening, and Subclinical Atherosclerotic Disease.Frontiers in cardiovascular medicine · 2019Review
- From stress to sight: The role of mechanical forces in the retinal diseases.Advances in ophthalmology practice and researchReview
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundVitreomacular adhesion (VMA) has been reported to associated with age-related macular degeneration (AMD). Understanding the mechanisms underlying cyclic stretch induced in retinal pigment epithelial cells (RPE) may be important for the treatment of VMA-related AMD.
methodCyclic stretch (1HZ, 20% elongation) was applied to cultured ARPE-19 cells for 15 min, 2 h, 6 h, 12 h, 24 h by flexcell FX-5000 Tension system. Total reactive oxygen species (ROS) were detected using DCFH-DA. Mitochondrial superoxide were detected using MitoSOX Red mitochondrial superoxide indicator. NADPH oxidases (NOX) and signaling pathways, such as p38 and PKC, were detected using western blot. Apocycin (Apo) were used as NOX inhibitors.
resultHigh levels of total ROS were detected from 15 min to 24 h, whereas mitochondrial superoxide were higher only in early time. NOX2 were significantly increased at 24 h. NOX4 were significantly increased at 2 h and reach its peak at 24 h. P-p38 was significantly increased at 12 h and 24 h. P-PKC was significantly increased at 15 min and kept a persistent high level. The upregulated expression of NOX4 by cyclic stretch can be significantly decreased under p-PKC inhibitor other than p-p38 inhibitor.
conclusionCyclic stretch induce oxidative stress from both mitochodrial and NADPH oxidase in RPE cells, which may prompt oxidative damage in VMA-related AMD.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.