Evidence map›Paper›PMID 30884320›Full record

ArticleBiomaterials2019

MiR-145 mediates cell morphology-regulated mesenchymal stem cell differentiation to smooth muscle cells.

Yi-Ting Yeh, Josh Wei, Satenick Thorossian, Katherine Nguyen, Clarissa Hoffman, Juan C Del Álamo, Ricardo Serrano, Yi-Shuan Julie Li, Kuei-Chun Wang, Shu Chien

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
3.1field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 45 citations in OpenAlex.

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  15. COPD-associated miR-145-5p is downregulated in early-decline FEVThe Journal of allergy and clinical immunology · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Yi-Ting YehDepartment of Bioengineering, University of California, San Diego, La Jolla, CA, 92093, United States; Institute of Engineering in Medicine, University of California, San Diego, La Jolla, CA, 92093, United States; Department of Mechanical and Aerospace Engineering, University of California, San Diego, La Jolla, CA, 92093, United States.
Josh WeiDepartment of Bioengineering, University of California, San Diego, La Jolla, CA, 92093, United States; Institute of Engineering in Medicine, University of California, San Diego, La Jolla, CA, 92093, United States.
Satenick ThorossianInstitute of Engineering in Medicine, University of California, San Diego, La Jolla, CA, 92093, United States.
Katherine NguyenDepartment of Bioengineering, University of California, San Diego, La Jolla, CA, 92093, United States; Institute of Engineering in Medicine, University of California, San Diego, La Jolla, CA, 92093, United States.
Clarissa HoffmanDepartment of Bioengineering, University of California, San Diego, La Jolla, CA, 92093, United States; Institute of Engineering in Medicine, University of California, San Diego, La Jolla, CA, 92093, United States.
Juan C Del ÁlamoInstitute of Engineering in Medicine, University of California, San Diego, La Jolla, CA, 92093, United States; Department of Mechanical and Aerospace Engineering, University of California, San Diego, La Jolla, CA, 92093, United States.
Ricardo SerranoDepartment of Mechanical and Aerospace Engineering, University of California, San Diego, La Jolla, CA, 92093, United States.
Yi-Shuan Julie LiDepartment of Bioengineering, University of California, San Diego, La Jolla, CA, 92093, United States; Institute of Engineering in Medicine, University of California, San Diego, La Jolla, CA, 92093, United States.
Kuei-Chun WangDepartment of Bioengineering, University of California, San Diego, La Jolla, CA, 92093, United States; Institute of Engineering in Medicine, University of California, San Diego, La Jolla, CA, 92093, United States. Electronic address: k9wang@ucsd.edu.
Shu ChienDepartment of Bioengineering, University of California, San Diego, La Jolla, CA, 92093, United States; Institute of Engineering in Medicine, University of California, San Diego, La Jolla, CA, 92093, United States. Electronic address: shuchien@eng.ucsd.edu.
University of California, San Diego · US

Funding

Systems Biology Analyses for Hemodynamic Regulation of Vascular HomeostasisR01HL108735 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CHIEN, SHU, SHYY, JOHN YJ · 2012 to 2024
$13.4M
Shear Regulation of MicroRNA Transportomes and Targetomes in Vascular HomeostasisR01HL106579 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CHIEN, SHU, SHYY, JOHN YJ · 2011 to 2022
$8.3M
Role of Spatiotemporal Epigenetic Dynamics in Regulating Endothelial Gene Expressions under FlowsR01HL121365 · NHLBI · UNIVERSITY OF SOUTHERN CALIFORNIA · PI CHIEN, SHU, WANG, YINGXIAO · 2014 to 2025
$7.5M
Flow Regulation of YAP/TAZ in Atherogenesis and Therapeutic DevelopmentR00HL135416 · NHLBI · ARIZONA STATE UNIVERSITY-TEMPE CAMPUS · PI WANG, MARK KUEI-CHUN · 2020 to 2022
$740k
Flow Regulation of YAP/TAZ in Atherogenesis and Therapeutic DevelopmentK99HL135416 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI WANG, MARK KUEI-CHUN · 2017 to 2018
$272k
NHLBI NIH HHS K99 HL135416NHLBI NIH HHS R00 HL135416NHLBI NIH HHS R01 HL106579NHLBI NIH HHS R01 HL108735NHLBI NIH HHS R01 HL121365
6 · The paper itself

Abstract

The use of biochemical signaling to derive smooth muscle cells (SMCs) from mesenchymal stem cells (MSCs) has been explored, but the induction of a fully functional SMC phenotype remains to be a major challenge. Cell morphology has been shown to regulate MSC differentiation into various lineages, including SMCs. We engineered substrates with microgrooves to induce cell elongation to study the mechanism underlying the MSC shape modulation in SMC differentiation. In comparison to those on flat substrates, MSCs cultured on engineered substrates were elongated with increased aspect ratios for both cell body and nucleus, as well as augmented cytoskeletal tensions. Biochemical studies indicated that the microgroove-elongated cells expressed significantly higher levels of SMC markers. MicroRNA analyses showed that up-regulation of miR-145 and the consequent repression of KLF4 in these elongated cells promoted MSC-to-SMC differentiation. Rho/ROCK inhibitions, which impair cytoskeletal tension, attenuated cell and nuclear elongations and disrupted the miR-145/KLF4 regulation for SMC differentiation. Furthermore, cell traction force measurements showed that miR-145 is essential for the functional contractility in the microgroove-induced SMC differentiation. Collectively, our findings demonstrate that, through a Rho-ROCK/miR-145/KLF4 pathway, the elongated cell shape serves as a decisive geometric cue to direct MSC differentiation into functional SMCs.

Indexed as

Cell DifferentiationCell ShapeBiomarkersDimethylpolysiloxanesHumansKruppel-Like Factor 4Kruppel-Like Transcription FactorsMesenchymal Stem CellsMicroRNAsModels, BiologicalMyocytes, Smooth MuscleSignal TransductionTransforming Growth Factor beta1Up-RegulationbaysilonBiomarkersDimethylpolysiloxanesKLF4 protein, humanKruppel-Like Factor 4Kruppel-Like Transcription FactorsMicroRNAsMIRN145 microRNA, humanTransforming Growth Factor beta1Cell shape modulationMesenchymal stem cellmiR-145Smooth muscle cell

Identifiers

PMID30884320
PMCPMC6825513
OpenAlexW2921009615

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.