Evidence map›Paper›PMID 30883703›Full record

ArticleBritish journal of pharmacology2019

Retinal and circulating miRNA expression patterns in diabetic retinopathy: An in silico and in vivo approach.

Chiara Bianca Maria Platania, Rosa Maisto, Maria Consiglia Trotta, Michele D'Amico, Settimio Rossi, Carlo Gesualdo, Giovanbattista D'Amico, Cornel Balta, Hildegard Herman, Anca Hermenean and 4 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in British journal of pharmacology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
84citing papers in PubMed, 2 pooled it
6.6field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

84 citing papers in PubMed, 2 syntheses or guidelines pooled it, 120 citations in OpenAlex.

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24 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 4 institutions in 2 countries.

Chiara Bianca Maria PlataniaDepartment of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Catania, Italy.
Rosa MaistoDepartment of Experimental Medicine, Division of Pharmacology, University of Campania "Luigi Vanvitelli", Naples, Italy.
Maria Consiglia TrottaDepartment of Experimental Medicine, Division of Pharmacology, University of Campania "Luigi Vanvitelli", Naples, Italy.
Michele D'AmicoDepartment of Experimental Medicine, Division of Pharmacology, University of Campania "Luigi Vanvitelli", Naples, Italy.
Settimio RossiEye Clinic, Multidisciplinary Department of Medical, Surgical and Dental Sciences, University of Campania "Luigi Vanvitelli", Naples, Italy.
Carlo GesualdoEye Clinic, Multidisciplinary Department of Medical, Surgical and Dental Sciences, University of Campania "Luigi Vanvitelli", Naples, Italy.
Giovanbattista D'AmicoInstitute of Life Sciences, Vasile Godis Western University of Arad, Arad, Romania.
Cornel BaltaInstitute of Life Sciences, Vasile Godis Western University of Arad, Arad, Romania.
Hildegard HermanInstitute of Life Sciences, Vasile Godis Western University of Arad, Arad, Romania.
Anca HermeneanInstitute of Life Sciences, Vasile Godis Western University of Arad, Arad, Romania.
Franca FerraraccioPathology Unit, Department of Mental and Physical Health and Preventive Medicine, University of Campania "Luigi Vanvitelli", Naples, Italy.
Iacopo PanaresePathology Unit, Department of Mental and Physical Health and Preventive Medicine, University of Campania "Luigi Vanvitelli", Naples, Italy.
Filippo DragoDepartment of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Catania, Italy.
Claudio BucoloDepartment of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Catania, Italy.ORCID 0000-0002-4879-4140
University of Campania "Luigi Vanvitelli" · ITUniversity of Catania · ITVasile Goldis Western University of Arad · ROUniversity of Bucharest · RO

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

background and purposeDiabetic retinopathy, a secondary complication of diabetes mellitus, can lead to irreversible vision loss. Currently, no treatment is approved for early phases of diabetic retinopathy. Modifications of the expression pattern of miRNAs could be involved in the early retinal damage of diabetic subjects. Therefore, we aimed at identification of dysregulated miRNAs-mRNA interactions that might be biomarkers and pharmacological targets for diagnosis and treatment of early diabetic retinopathy.

methodsA focused set of miRNAs was predicted through a bioinformatic analysis accessing to Gene Expression Omnibus dataset and enrichment of information approach (GENEMANIA-Cytoscape). Identification of miRNAs-mRNA interactions was carried out with miRNET analysis. Diabetes was induced in C57BL6J mice by streptozotocin and samples analysed at 5 and 10 weeks after diabetes induction. Retinal ultrastructure of diabetic mice was analysed through electron microscopy. We used Real-time PCR, western blot analysis, elisa, and immunohistochemistry to study expression of miRNAs and possible targets of dysregulated miRNAs. KEY

resultsWe found that miR-20a-5p, miR-20a-3p, miR-20b, miR-106a-5p, miR-27a-5p, miR-27b-3p, miR-206-3p, and miR-381-3p were dysregulated in the retina and serum of diabetic mice. VEGF, brain-derived neurotrophic factor (BDNF), PPAR-α, and cAMP response element-binding protein 1 (CREB1) are targets of dysregulated miRNAs, which then modulated protein expression in diabetic retina. We found structural modifications in retinas from diabetic mice. CONCLUSIONS AND IMPLICATIONS: Serum and retina of diabetic mice express eight dysregulated miRNAs, which modified the expression of VEGF, BDNF, PPAR-α, and CREB1, before vasculopathy in diabetic retinas.

Indexed as

AnimalsBrain-Derived Neurotrophic FactorComputer SimulationCyclic AMP Response Element-Binding ProteinDiabetes Mellitus, ExperimentalDiabetic RetinopathyMaleMice, Inbred C57BLMicroRNAsPPAR alphaRetinaVascular Endothelial Growth Factor ABdnf protein, mouseBrain-Derived Neurotrophic FactorCreb1 protein, mouseCyclic AMP Response Element-Binding ProteinMicroRNAsPPAR alphaPpara protein, mouseVascular Endothelial Growth Factor Avascular endothelial growth factor A, mouse

Identifiers

PMID30883703
PMCPMC6555853
OpenAlexW2924850948

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.