ArticleCurrent protocols in neuroscience2019
Production of Viral Constructs for Neuroanatomy, Calcium Imaging, and Optogenetics.
Article in Current protocols in neuroscience, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed.
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- α7 nicotinic receptor activation mitigates herpes simplex virus type 1 infection in microglia cells.Antiviral research · 2024Article
- Nucleolar Architecture Is Modulated by a Small Molecule, the Inositol Pyrophosphate 5-InsPBiomolecules · 2023Article
- Longitudinal Serological Surveillance for COVID-19 Antibodies after Infection and Vaccination.Microbiology spectrum · 2022Observational
- Imaging Inflammasome Activation in Microglia.Current protocols · 2022Article
- Nebulized delivery of a broadly neutralizing SARS-CoV-2 RBD-specific nanobody prevents clinical, virological and pathological disease in a Syrian hamster model of COVID-19.bioRxiv : the preprint server for biology · 2021Article
- High affinity nanobodies block SARS-CoV-2 spike receptor binding domain interaction with human angiotensin converting enzyme.Scientific reports · 2020Article
- AAV diffuses across zona pellucida for effortless gene delivery to fertilized eggs.Biochemical and biophysical research communications · 2020Article
- In Vivo Two-photon Calcium Imaging in Dendrites of Rabies Virus-labeled V1 Corticothalamic Neurons.Neuroscience bulletin · 2020Article
- Automated Live-Cell Imaging of Synapses in Rat and Human Neuronal Cultures.Frontiers in cellular neuroscience · 2019Article
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Authors and funding
6 authors.
Funding
Abstract
Advances in design and use of light-sensitive and light-emitting sensors have facilitated observation, measurement, and control of neuronal activities. Viruses are effective vectors for delivery of these valuable research tools to mammalian brains. Recombinant viruses are optimized to mediate regulatable, long-term, and cell-specific gene expression. Here, we describe production methods for three of the most commonly used types of recombinant viruses in neurobiology research: adeno-associated virus (AAV), retrovirus/lentivirus, and glycoprotein-deleted rabies virus. These viral constructs are frequently used for calcium imaging or to deliver neural tracers and optogenetic tools. Popular constructs are readily obtained commercially; however, customized virus production through commercial sources is time consuming and costly. This article aims to provide readers with detailed technical information for rapid production and validation of high-quality viral particles in a laboratory setting while highlighting advantages and limitations of each viral type. © 2019 by John Wiley & Sons, Inc.
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