ArticleJournal of visualized experiments : JoVE2019
Erratum: A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes.
Article in Journal of visualized experiments : JoVE, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
An erratum was issued for: A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes. There were typos in the Protocol section and Table 2. Step 3.1.1 in the Protocol was updated from: Add 80 µl of 1 mM Pefa Bloc and 60 mg of octyl-β-D-glucopyranaside to 3,920 µl of Citrate Phosphate Buffer. (See supplemental spreadsheet calculator for alternative scaling). to: Add 80 µl of 1 mM Pefa Bloc and 60 µg of octyl-β-D-glucopyranaside to 3,920 µl of Citrate Phosphate Buffer. (See supplemental spreadsheet calculator for alternative scaling). Step 5.2 in the Protocol was updated from: Further dilute the Control Peptide from step 5.1 (20 mM) 1:100 into the MHC II Master Mix from step 3.2. to: Further dilute the Control Peptide from step 5.1 (20 µM) 1:100 into the MHC II Master Mix from step 3.2. Table 2 was updated from: MHC II Allele DRB1*: 1501 MHC II Stock Concentration (mg/ml) 1.3 MHC II Stock Concentration (mM) 20 Vol. MHC II Stock to Add (ml) 14.65 Vol. Reaction Buffer to Add (ml): 2885.35 MHC II Master Mix Concentration (nM) 101 to: MHC II Allele DRB1*: 1501 MHC II Stock Concentration (mg/ml) 1.3 MHC II Stock Concentration (μM) 20 Vol. MHC II Stock to Add (μl) 14.65 Vol. Reaction Buffer to Add (μl): 2885.35 MHC II Master Mix Concentration (nM) 101.
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