ArticleChemical science2019
An integrated-molecular-beacon based multiple exponential strand displacement amplification strategy for ultrasensitive detection of DNA methyltransferase activity.
Article in Chemical science, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 60 citations in OpenAlex.
- Droplet digital molecular beacon-LAMP assay via pico-injection for ultrasensitive detection of pathogens.Mikrochimica acta · 2024Article
- Three-input logic gate based on DNA strand displacement reaction.Scientific reports · 2023Article
- Isothermal nucleic acid amplification and its uses in modern diagnostic technologies.3 Biotech · 2023Review
- Development of the DNA-based biosensors for high performance in detection of molecular biomarkers: More rapid, sensitive, and universal.Biosensors & bioelectronics · 2022Review
- Programmable High-Speed and Hyper-Efficiency DNA Signal Magnifier.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Recent advances of fluorescent biosensors based on cyclic signal amplification technology in biomedical detection.Journal of nanobiotechnology · 2021Review
- Cascade Strand Displacement and Bipedal Walking Based DNA Logic System for miRNA Diagnostics.ACS central science · 2021Article
- First passage time study of DNA strand displacement.Biophysical journal · 2021Article
- Ultrasensitive DNA methyltransferase activity sensing and inhibitor evaluation with highly photostable upconversion nanoparticle transducer.Mikrochimica acta · 2021Article
- A controlled T7 transcription-driven symmetric amplification cascade machinery for single-molecule detection of multiple repair glycosylases.Chemical science · 2021Article
- Graphene oxide assisted light-up aptamer selection against Thioflavin T for label-free detection of microRNA.Scientific reports · 2021Article
- Cytosine-5 methylation-directed construction of a Au nanoparticle-based nanosensor for simultaneous detection of multiple DNA methyltransferases at the single-molecule level.Chemical science · 2020Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA methylation is a significant epigenetic mechanism involving processes of transferring a methyl group onto cytosine or adenine. Such DNA modification catalyzed by methyltransferase (MTase) plays important roles in the modulation of gene expression and other cellular activities. Herein, we develop a simple and sensitive biosensing platform for the detection of DNA MTase activity by using only two oligonucleotides. The fluorophore labeled molecular beacon (MB) can be methylated by MTase and subsequently cleaved by endonuclease DpnI at the stem, giving a shortened MB. The shortened MB can then hybridize with a primer DNA, initiating a cycle of strand displacement amplification (SDA) reactions. The obtained SDA products can unfold new MB and initiate another cycle of SDA reaction. Therefore, continuous enlargement of SDA and exponential amplification of the fluorescence signal are achieved. Because the triple functions of substrate, template and probe are elegantly integrated in one oligonucleotide, only two oligonucleotides are necessary for multiple amplification cycles, which not only reduces the complexity of the system, but also overcomes the laborious and cumbersome operation that is always a challenge in conventional methods. This platform exhibits an extremely low limit of detection of 3.3 × 10
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