ArticleFrontiers in genetics2019
Pan-Cancer Analysis of TCGA Data Revealed Promising Reference Genes for qPCR Normalization.
Article in Frontiers in genetics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.
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Who cites it
35 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Selection of reference genes for quantitative PCR: identifying reference genes for airway epithelial cells exposed toAmerican journal of physiology. Lung cellular and molecular physiology · 2020Pooled it
- Nuclear adenosine metabolism defines a metabolic vulnerability unmasked by TP53 loss.bioRxiv : the preprint server for biology · 2026Article
- Advances in algorithms for normalizer gene selection in qRT-PCR: implications for cancer biology and precision medicine.Frontiers in genetics · 2026Review
- Selecting Optimal Housekeeping Genes for RT-qPCR in Endometrial Cancer Studies: A Narrative Review.International journal of molecular sciences · 2025Review
- Integrative Genomic and Functional Approaches Identify FUOM as a Key Driver and Therapeutic Target in Cervical Cancer.Cancer reports (Hoboken, N.J.) · 2025Article
- Prognostic and predictive impact of NOTCH1 in early breast cancer.Breast cancer research and treatment · 2025Article
- Identification of endogenous reference genes for RT-qPCR analysis in breast cancer and matched adjacent tissues.Frontiers in oncology · 2025Article
- Techniques for assessing telomere length: A methodological review.Computational and structural biotechnology journal · 2024Review
- The Notable Role of Telomere Length Maintenance in Complex Diseases.Biomedicines · 2024Review
- Ten Hypermethylated lncRNA Genes Are Specifically Involved in the Initiation, Progression, and Lymphatic and Peritoneal Metastasis of Epithelial Ovarian Cancer.International journal of molecular sciences · 2024Article
- Neutrophil Elastase Remodels Mammary Tumors to Facilitate Lung Metastasis.Molecular cancer therapeutics · 2024Article
- Epigenetic reprogramming of a distal developmental enhancer cluster drives SOX2 overexpression in breast and lung adenocarcinoma.Nucleic acids research · 2023Article
- Mitochondrial UQCC3 controls embryonic and tumor angiogenesis by regulating VEGF expression.iScience · 2023Article
- GAPDH: A common housekeeping gene with an oncogenic role in pan-cancer.Computational and structural biotechnology journal · 2023Article
- Experimental assessment of robust reference genes for qRT-PCR in lung cancer studies.Frontiers in oncology · 2023Article
- Article
- Aberrant Methylation of 20 miRNA Genes Specifically Involved in Various Steps of Ovarian Carcinoma Spread: From Primary Tumors to Peritoneal Macroscopic Metastases.International journal of molecular sciences · 2022Article
- Integration of artificial intelligence and precision oncology in Latin America.Frontiers in medical technology · 2022Review
- Public transcriptome database-based selection and validation of reliable reference genes for breast cancer research.Biomedical engineering online · 2021Article
- Identification of Novel Endogenous Controls for qPCR Normalization in SK-BR-3 Breast Cancer Cell Line.Genes · 2021Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quantitative PCR (qPCR) remains the most widely used technique for gene expression evaluation. Obtaining reliable data using this method requires reference genes (RGs) with stable mRNA level under experimental conditions. This issue is especially crucial in cancer studies because each tumor has a unique molecular portrait. The Cancer Genome Atlas (TCGA) project provides RNA-Seq data for thousands of samples corresponding to dozens of cancers and presents the basis for assessment of the suitability of genes as reference ones for qPCR data normalization. Using TCGA RNA-Seq data and previously developed CrossHub tool, we evaluated mRNA level of 32 traditionally used RGs in 12 cancer types, including those of lung, breast, prostate, kidney, and colon. We developed an 11-component scoring system for the assessment of gene expression stability. Among the 32 genes,
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