Evidence map›Paper›PMID 30880156›Full record

ArticleCell chemical biology2019

Tracing MYC Expression for Small Molecule Discovery.

Jutta Steinberger, Francis Robert, Maxime Hallé, David E Williams, Regina Cencic, Neha Sawhney, Dylan Pelletier, Philip Williams, Yasuhiro Igarashi, John A Porco and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell chemical biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Decoding thebioRxiv : the preprint server for biology · 2026
    Article
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Jutta SteinbergerDepartment of Biochemistry, McGill University, Montreal, QC H3G 1Y6, Canada.
Francis RobertDepartment of Biochemistry, McGill University, Montreal, QC H3G 1Y6, Canada.
Maxime HalléDepartment of Biochemistry, McGill University, Montreal, QC H3G 1Y6, Canada.
David E WilliamsDepartments of Chemistry and Earth, Ocean and Atmospheric Sciences, University of British Columbia, Vancouver, BC V6T 1Z1, Canada.
Regina CencicDepartment of Biochemistry, McGill University, Montreal, QC H3G 1Y6, Canada.
Neha SawhneyVanderbilt Institute of Chemical Biology, Department of Chemistry, Vanderbilt University, Nashville, 37235, USA.
Dylan PelletierDepartment of Biochemistry, McGill University, Montreal, QC H3G 1Y6, Canada.
Philip WilliamsDepartment of Chemistry, University of Hawaii at Manoa, Honolulu, HI 96822, USA.
Yasuhiro IgarashiBiotechnology Research Center, Toyama Prefectural University, Toyama 939-0398, Japan.
John A PorcoDepartment of Chemistry, Center for Molecular Discovery (BU-CMD), Boston University, Boston, MA 02215, USA.
Abimael D RodriguezMolecular Sciences Research Center, University of Puerto Rico, San Juan, PR 00926, USA.
Brigitte KoppDepartment of Pharmacognosy, University of Vienna, Vienna, Austria.
Brian BachmannVanderbilt Institute of Chemical Biology, Department of Chemistry, Vanderbilt University, Nashville, 37235, USA.
Raymond J AndersenDepartments of Chemistry and Earth, Ocean and Atmospheric Sciences, University of British Columbia, Vancouver, BC V6T 1Z1, Canada.
Jerry PelletierDepartment of Biochemistry, McGill University, Montreal, QC H3G 1Y6, Canada; The Rosalind and Morris Goodman Cancer Research Center and the Department of Oncology, McGill University, Montreal, QC, Canada. Electronic address: jerry.pelletier@mcgill.ca.

Funding

Chemical Synthesis of Complex Natural Products for Translational ScienceR35GM118173 · NIGMS · BOSTON UNIVERSITY (CHARLES RIVER CAMPUS) · PI JOHN A. PORCO · 2016 to 2026
$8.2M
Single Cell Methods for Bioeffector Discovery and AnalysisR01CA226833 · NCI · VANDERBILT UNIVERSITY · PI BRIAN O BACHMANN, Jonathan Michael Irish · 2019 to 2026
$6.1M
New Methodologies for Accelerating Natural Product DiscoveryR01GM092218 · NIGMS · VANDERBILT UNIVERSITY · PI BACHMANN, BRIAN O, MCLEAN, JOHN ALAN · 2010 to 2018
$2.8M
Center for Molecular Discovery (CMD): A Small Molecule Resource for Biomedical ResearchR24GM111625 · NIGMS · BOSTON UNIVERSITY (CHARLES RIVER CAMPUS) · PI PORCO, JOHN A. · 2015 to 2017
$1.5M
New Leads Against Cancer and Infectious Diseases from Puerto Rican Reef SpeciesSC1GM086271 · NIGMS · UNIVERSITY OF PUERTO RICO RIO PIEDRAS · PI RODRIGUEZ, ABIMAEL D · 2010 to 2013
$1.2M
CIHR FDN-148366NCI NIH HHS R01 CA226833NIGMS NIH HHS R01 GM092218NIGMS NIH HHS R24 GM111625NIGMS NIH HHS R35 GM118173NIGMS NIH HHS SC1 GM086271
6 · The paper itself

Abstract

Our inability to effectively "drug" targets such as MYC for therapeutic purposes requires the development of new approaches. We report on the implementation of a phenotype-based assay for monitoring MYC expression in multiple myeloma cells. The open reading frame (ORF) encoding an unstable variant of GFP was engineered immediately downstream of the MYC ORF using CRISPR/Cas9, resulting in co-expression of both proteins from the endogenous MYC locus. Using fluorescence readout as a surrogate for MYC expression, we implemented a pilot screen in which ∼10,000 compounds were prosecuted. Among known MYC expression inhibitors, we identified cardiac glycosides and cytoskeletal disruptors to be quite potent. We demonstrate the power of CRISPR/Cas9 engineering in establishing phenotype-based assays to identify gene expression modulators.

Indexed as

BufanolidesCardiac GlycosidesCell Line, TumorCell SurvivalCRISPR-Cas SystemsCytoskeletonEukaryotic Initiation Factor-2HumansProto-Oncogene Proteins c-mycRNA InterferenceRNA, Small InterferingSmall Molecule LibrariesTranscription, GeneticbufalinBufanolidesCardiac GlycosidesEukaryotic Initiation Factor-2Proto-Oncogene Proteins c-mycRNA, Small InterferingSmall Molecule Librarieschemical biologyCRISPR/Cas9drug discoverydrug repurposinggenome engineeringmultiple myelomasMYC expressionphenotype-based assaytarget identification

Identifiers

PMID30880156
PMCPMC6525070

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.