ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2019
Synthetic Antigen Gels as Practical Controls for Standardized and Quantitative Immunohistochemistry.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 11 citations in OpenAlex.
- Next-generation sequencing elucidates the distinct mutational landscape of SMARCA4-deficient lung cancer.Oncology letters · 2026Article
- High-throughput histopathology for complex in vitro models.Cell reports methods · 2026Article
- Practical quantification of immunohistochemistry antigen concentrations and reaction-diffusion parameters.bioRxiv : the preprint server for biology · 2026Article
- Quantitative assessment of H&E staining for pathology: development and clinical evaluation of a novel system.Diagnostic pathology · 2024Article
- Seeing or believing in hyperplexed spatial proteomics via antibodies: New and old biases for an image-based technology.Biological imaging · 2024Article
- Development of novel quality control material based on CRISPR/Cas9 editing and xenografts for MLH1 protein deficiency testing.Journal of clinical laboratory analysis · 2021Article
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Authors and funding
7 authors.
Funding
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Abstract
Optimization and standardization of immunohistochemistry (IHC) protocols within and between laboratories requires reproducible positive and negative control samples. In many situations, suitable tissue or cell line controls are not available. We demonstrate here a method to incorporate target antigens into synthetic protein gels that can serve as IHC controls. The method can use peptides, protein domains, or whole proteins as antigens, and is compatible with a variety of fixation protocols. The resulting gels can be used to create tissue microarrays (TMAs) with a range of antigen concentrations that can be used to objectively quantify and calibrate chromogenic, fluorescent, or mass spectrometry-based IHC protocols. The method offers an opportunity to objectively quantify IHC staining results, and to optimize and standardize IHC protocols within and between laboratories. (J Histochem Cytochem 58:XXX-XXX, 2019).
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Registered trials
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