Evidence map›Paper›PMID 30879273›Full record

ArticleMolecular biology reports2019

In silico prediction of short hairpin RNA and in vitro silencing of activin receptor type IIB in chicken embryo fibroblasts by RNA interference.

P Guru Vishnu, T K Bhattacharya, Bharat Bhushan, Pushpendra Kumar, R N Chatterjee, Chandan Paswan, K Dushyanth, D Divya, A Rajendra Prasad

Abstract read
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In one paragraph

Article in Molecular biology reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.7field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 10 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

P Guru VishnuSri Venkateswara Veterinary University, Tirupathi, A.P., India. vishnugenetics@gmail.com.ORCID http://orcid.org/0000-0002-6361-1189
T K BhattacharyaICAR-Directorate of Poultry Research, Hyderabad, India.
Bharat BhushanDivision of Animal Genetics & Breeding, Indian Veterinary Research Institute, Izatnagar, U.P., India.
Pushpendra KumarDivision of Animal Genetics & Breeding, Indian Veterinary Research Institute, Izatnagar, U.P., India.
R N ChatterjeeICAR-Directorate of Poultry Research, Hyderabad, India.
Chandan PaswanICAR-Directorate of Poultry Research, Hyderabad, India.
K DushyanthICAR-Directorate of Poultry Research, Hyderabad, India.
D DivyaICAR-Directorate of Poultry Research, Hyderabad, India.
A Rajendra PrasadDivision of Animal Genetics & Breeding, Indian Veterinary Research Institute, Izatnagar, U.P., India.
Directorate of Poultry Research · INIndian Veterinary Research Institute · INSri Venkateswara University · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Gene silencing by RNA interference is extensively used reverse genetic approach to analyse the implications of any gene in mammalian systems. The silencing of the Activin type IIB receptor belonging to transforming growth factor beta superfamily has demonstrated increase in muscle growth in many species. We designed five short hairpin RNA constructs targeting coding region of chicken ACTRIIB. All the shRNAs were transfected into chicken embryo fibroblast cells and evaluated their silencing efficiency by real time PCR and western blotting. Initially the computational analysis of target region and shRNA constructs was undertaken to predict sequence based features (secondary structures, GC% and H-b index) and thermodynamic features (ΔG

Indexed as

Activin Receptors, Type IIAnimalsChick EmbryoChickensComputer SimulationEmbryo, MammalianFibroblastsGene SilencingMuscle DevelopmentRNA InterferenceRNA, MessengerRNA, Small InterferingTransfectionActivin Receptors, Type IIactivin receptor type II-BRNA, MessengerRNA, Small InterferingACTRIIBGene expressionGene silencingMuscle growthSecondary structuresThermodynamic parameters

Identifiers

PMID30879273
OpenAlexW2921902114

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.