Evidence map›Paper›PMID 30877395›Full record

ArticleMikrochimica acta2019

Colorimetric detection of nucleic acid sequences in plant pathogens based on CRISPR/Cas9 triggered signal amplification.

Weidan Chang, Weipeng Liu, Ying Liu, Fangfang Zhan, Huifang Chen, Hongtao Lei, Yingju Liu

Abstract read
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In one paragraph

Article in Mikrochimica acta, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
3.0field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 69 citations in OpenAlex.

  1. Review
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  4. Article
  5. Review
  6. Next-Generation Diagnostic with CRISPR/Cas: Beyond Nucleic Acid Detection.International journal of molecular sciences · 2022
    Review
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  8. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Weidan ChangCollege of Materials & Energy, South China Agricultural University, Guangzhou, 510642, China.
Weipeng LiuCollege of Materials & Energy, South China Agricultural University, Guangzhou, 510642, China.
Ying LiuCollege of Materials & Energy, South China Agricultural University, Guangzhou, 510642, China.
Fangfang ZhanFujian Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Huifang ChenCollege of Materials & Energy, South China Agricultural University, Guangzhou, 510642, China.
Hongtao LeiThe Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou, 510642, China.
Yingju LiuCollege of Materials & Energy, South China Agricultural University, Guangzhou, 510642, China. liuyingju@hotmail.com.
South China Agricultural University · CNFujian Agriculture and Forestry University · CN

Funding

National Natural Science Foundation of China 21705051National Natural Science Foundation of China 21874048
6 · The paper itself

Abstract

A colorimetric method is presented for the detection of specific nucleotide sequences in plant pathogens. It is based on the use of CRISPR/Cas9-triggered isothermal amplification and gold nanoparticles (AuNPs) as optical probes. The target DNA was recognized and broken up by a given Cas9/sgRNA complex. After isothermal amplification, the product was hybridized with oligonucleotide-functionalized AuNPs. This resulted in the aggregation of AuNPs and a color change from wine red to purple. The visual detection limit is 2 pM of DNA, while a linear relationship exists between the ratio of absorbance at 650 and 525 nm and the DNA concentration in the range from 0.2 pM to 20 nM. In contrast to the previous CRISPR-based amplification platforms, the method has significantly higher specificity with the single-base mismatch and can be visually read out. It was successfully applied to identify the Phytophthora infestans genomic DNA. Graphical abstract Schematic presentation of a colorimetric method for detection of Phytophthora infestans genomic DNA based on CRISPR/Cas9-triggered isothermal amplification. The Cas9 endonuclease cleaves DNA at the design site and the color changes from red to purple with increasing target DNA concentration.

Indexed as

CRISPR-Cas SystemsBase SequenceBiosensing TechniquesColorimetryCRISPR-Associated Protein 9DNAGoldLimit of DetectionMetal NanoparticlesNucleic Acid Amplification TechniquesNucleic Acid HybridizationOligodeoxyribonucleotidesPhytophthora infestansCRISPR-Associated Protein 9DNAGoldOligodeoxyribonucleotidesAuNP probesCas9/sgRNA complexDouble-strand breakGold nanoparticlesIsothermal amplificationLocalized surface plasmon resonancePhytophthora infestansRolling circle amplificationSingle-base mismatchTriggered aggregation

Identifiers

PMID30877395
OpenAlexW2921110444

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.