ArticleApplied microbiology and biotechnology2019
Generation, identification, and functional analysis of monoclonal antibodies against porcine epidemic diarrhea virus nucleocapsid.
Article in Applied microbiology and biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 35 citations in OpenAlex.
- Identification of Novel B Cell Epitopes on the Nucleocapsid Protein of Porcine Epidemic Diarrhea Virus.Viruses · 2026Article
- A novel double-antibody sandwich ELISA based on monoclonal antibodies against the viral spike protein detects porcine deltacoronavirus infection.Microbiology spectrum · 2025Article
- Advances in porcine epidemic diarrhea virus research: genome, epidemiology, vaccines, and detection methods.Discover nano · 2025Review
- Establishment and application of a quadruple RT-qPCR method for simultaneous detection of porcine enteric coronaviruses.Frontiers in veterinary science · 2025Article
- High-affinity monoclonal antibodies against the porcine epidemic diarrhea virus S1 protein.BMC veterinary research · 2024Article
- Preparation and application of fluorescent monoclonal antibodies recognizing goat CD4Applied microbiology and biotechnology · 2024Article
- Article
- Linoleic acid: a natural feed compound against porcine epidemic diarrhea disease.Journal of virology · 2023Article
- Article
- Visual and Ultrasensitive Detection of a Coronavirus Using a Gold Nanorod Probe under Dark Field.Biosensors · 2022Article
- Characterisation of ORF3, M, N and E Gene Sequences of Porcine Epidemic Diarrhoea Virus from Domestic Pigs in Poland.Journal of veterinary research · 2022Article
- Generation and functional analysis of single chain variable fragments (scFvs) targeting the nucleocapsid protein of Porcine epidemic diarrhea virus.Applied microbiology and biotechnology · 2022Article
- Article
- Identification and characterization of PEDV infection in rat crypt epithelial cells.Veterinary microbiology · 2020Article
- Preparation, identification, and functional analysis of monoclonal antibodies against atypical porcine pestivirus NS3 protein.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2020Article
- Identification of a Novel Linear B-Cell Epitope on the Nucleocapsid Protein of Porcine Deltacoronavirus.International journal of molecular sciences · 2020Article
- A TaqMan-probe-based multiplex real-time RT-qPCR for simultaneous detection of porcine enteric coronaviruses.Applied microbiology and biotechnology · 2019Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
The variant strains of porcine epidemic diarrhea virus (PEDV) severely threaten the pig industry worldwide and cause up to 100% mortality in suckling piglets. It is critically important and urgent to develop tools for detection of PEDV infection. In this study, we developed six monoclonal antibodies (mAbs) targeting N protein of PEDV and analyzed their applications on enzyme-linked immunosorbent assay (ELISA), indirect immunofluorescence assay (IFA), western blot assay, and flow cytometry assay. The results demonstrated that all these six mAbs were IgG1 isotype and κ chain. Among these six mAbs, 3F12 recognizes a linear epitope (VAAVKDALKSLGI) while the other five mAbs recognize different conformational epitopes formed by a specific peptide fragment or the full length of N protein. The functional analysis showed that all these six mAbs were applicable to ELISA, western blot, IFA, and flow cytometry assay. In conclusion, we developed six mAbs against PEDV-N protein to facilitate the early detection of PEDV infection using ELISA, western blot, IFA, and flow cytometry.
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