ArticleApplied microbiology and biotechnology2019
Haloferax volcanii as immobilised whole cell biocatalyst: new applications for halophilic systems.
Article in Applied microbiology and biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 25 citations in OpenAlex.
- Ultrasound Imaging Properties of Heterologously Synthesized Gas Vesicles from Halophilic Archaeon.Nanomaterials (Basel, Switzerland) · 2025Article
- Review
- Advanced Strategies for Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Production: PHA Synthase Homologous Overexpression in the ExtremophileMarine drugs · 2025Article
- Haloarchaea as Promising Chassis to Green Chemistry.Microorganisms · 2024Review
- Analysis of Lsm Protein-Mediated Regulation in the HaloarchaeonInternational journal of molecular sciences · 2024Article
- A systematic analysis of affinity tags in the haloarchaeal expression system,Frontiers in microbiology · 2024Article
- Bioengineering of air-filled protein nanoparticles by genetic and chemical functionalization.Journal of nanobiotechnology · 2023Article
- Genetic Manipulation of Haloferax Species.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Ubiquitousness ofMarine drugs · 2021Article
- Analysis ofGenes · 2021Article
- Review
- Haloferax volcanii for biotechnology applications: challenges, current state and perspectives.Applied microbiology and biotechnology · 2020Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Enzyme-mediated synthesis of pharmaceutical compounds is a 'green' alternative to traditional synthetic chemistry, and microbial engineering opens up the possibility of using whole cells as mini-factories. Whole-cell biocatalysis reduces cost by eliminating expensive enzyme purification and cofactor addition steps, as well as resulting in increased enzyme stability. Haloferax volcanii is a model halophilic archaeon encoding highly salt and organic solvent tolerant enzymes such as alcohol dehydrogenase (HvADH2), which catalyses the reduction of aldehydes and ketone in the presence of NADPH/NADH cofactor. A H. volcanii strain for constitutive HvADH2 expression was generated using a strong synthetic promoter (p.syn). The strain was immobilised in calcium alginate beads and repeatedly used as a whole-cell biocatalyst. The reduction of acetophenone, used as test substrate, was very successful and high yields were detected from immobilised whole cells over repeated biotransformation cycles. The immobilised H. volcanii retained stability and high product yields after 1 month of storage at room temperature. This newly developed system offers halophilic enzyme expression in its native environment, high product yield, stability and reusability without the addition of any expensive NADPH/NADH cofactor. This is the first report of whole cell-mediated biocatalysis by the halophilic archaeon H. volcanii.
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