ArticlePLoS genetics2019
The dynamin-like protein Fzl promotes thylakoid fusion and resistance to light stress in Chlamydomonas reinhardtii.
Article in PLoS genetics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 41 citations in OpenAlex.
- The Spirogyra pratensis genome illuminates the evolution of developmental programs and spiral chloroplast biology.The Plant cell · 2026Article
- CRISPR-Cas Technology TurnsMarine drugs · 2025Review
- Thylakostasis: key factors in thylakoid membrane organization with emphasis on biogenesis and remodeling proteins in vascular plants.Plant & cell physiology · 2025Review
- Cyanoglobule lipid droplets are a stress-responsive metabolic compartment of cyanobacteria and the progenitor of plant plastoglobules.The Plant cell · 2025Article
- Structure, biogenesis, and evolution of thylakoid membranes.The Plant cell · 2024Review
- Role of isotropic lipid phase in the fusion of photosystem II membranes.Photosynthesis research · 2024Article
- Dramatic Changes in Mitochondrial Subcellular Location and Morphology Accompany Activation of the CObioRxiv : the preprint server for biology · 2024Article
- Biomanufacturing of γ-linolenic acid-enriched galactosyldiacylglycerols: Challenges in microalgae and potential in oleaginous yeasts.Synthetic and systems biotechnology · 2023Review
- Chlamydomonas mutants lacking chloroplast TRIOSE PHOSPHATE TRANSPORTER3 are metabolically compromised and light sensitive.The Plant cell · 2023Article
- A Novel Group of Dynamin-Related Proteins Shared by Eukaryotes and Giant Viruses Is Able to Remodel Mitochondria From Within the Matrix.Molecular biology and evolution · 2023Article
- SynDLP is a dynamin-like protein of Synechocystis sp. PCC 6803 with eukaryotic features.Nature communications · 2023Article
- FZL, a dynamin-like protein localized to curved grana edges, is required for efficient photosynthetic electron transfer inFrontiers in plant science · 2023Article
- Systematic characterization of gene function in the photosynthetic alga Chlamydomonas reinhardtii.Nature genetics · 2022Article
- A glossary of plant cell structures: Current insights and future questions.The Plant cell · 2022Review
- Orchestral manoeuvres in the light: crosstalk needed for regulation of the Chlamydomonas carbon concentration mechanism.Journal of experimental botany · 2021Review
- Genome Editing by CRISPR-Cas: A Game Change in the Genetic Manipulation ofLife (Basel, Switzerland) · 2020Review
- Proton Leakage Is Sensed by IM30 and Activates IM30-Triggered Membrane Fusion.International journal of molecular sciences · 2020Article
- TIM, a targeted insertional mutagenesis method utilizing CRISPR/Cas9 in Chlamydomonas reinhardtii.PloS one · 2020Article
- Genetic Engineering Strategies forFrontiers in bioengineering and biotechnology · 2020Review
- Electron Tomography Analysis of Thylakoid Assembly and Fission in Chloroplasts of a Single-Cell C4 plant, Bienertia sinuspersici.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Large GTPases of the Dynamin Related Proteins (DRP) family shape lipid bilayers through membrane fission or fusion processes. Despite the highly organized photosynthetic membranes of thylakoids, a single DRP is known to be targeted inside the chloroplast. Fzl from the land plant Arabidopsis thaliana is inserted in the inner envelope and thylakoid membranes to regulate their morphology. Fzl may promote the fusion of thylakoids but this remains to be proven. Moreover, the physiological requirement for fusing thylakoids is currently unknown. Here, we find that the unicellular microalga Chlamydomonas reinhardtii encodes an Fzl ortholog (CrFzl) that is localized in the chloroplast where it is soluble. To explore its function, the CRISPR/Cas9 technology was employed to generate multiple CrFzl knock out strains. Phenotypic analyzes revealed a specific requirement of CrFzl for survival upon light stress. Consistent with this, strong irradiance lead to increased photoinhibition of photosynthesis in mutant cells. Fluorescence and electron microscopy analysis demonstrated that upon exposure to high light, CrFzl mutants show defects in chloroplast morphology but also large cytosolic vacuoles in close contact with the plastid. We further observe that strong irradiance induces an increased recruitment of the DRP to thylakoid membranes. Most importantly, we show that CrFzl is required for the fusion of thylakoids during mating. Together, our results suggest that thylakoids fusion may be necessary for resistance to light stress.
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