ArticleTurkish journal of urology2019
Fibronectin as a new biomarker for human sperm selection in assisted reproductive technology.
Article in Turkish journal of urology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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Who cites it
6 citing papers in PubMed, 12 citations in OpenAlex.
- Dietary glycemic index, glycemic load, carbohydrate quality index, and low-carbohydrate diet score and their association with sperm DNA damage in unexplained male infertility.Genes & nutrition · 2026Article
- Dietary patterns and sperm DNA fragmentation in idiopathic infertile men: A case-control study.Scientific reports · 2026Article
- Dietary Arginine Supplementation Modulates the Proteome of Boar Seminal Plasma.Animals : an open access journal from MDPI · 2025Article
- Comparison of Embryological Results of Microinjection in Two Groups of Men with and without Requesting Sperm DNA Fragmentation Index Measurement.BioMed research international · 2024Article
- Proteomic Landscape of Human Sperm in Patients with Different Spermatogenic Impairments.Cells · 2023Article
- Comparative Analysis of the Effects of Magnesium Oxide Nanoparticles on Sperm Parameters in Fresh and Frozen Samples.Journal of reproduction & infertilityArticle
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveFibronectin (FN) is a multifunctional diametric glycoprotein on the surface of the sperm that plays an important role in the sperm-oocyte interaction and fertilization process. The aim of the present study was to assess the FN levels as a sperm surface biomarker for sperm selection in assisted reproductive technology. MATERIAL AND
methodsPolyclonal antibody against human FN was produced in rabbit. Its quality, purity, and immune reactivity were assessed by SDS-PAGE and western blot. In addition, the presence of FN on the sperm surface was assessed through immunocytochemistry and flow cytometry. The amount of FN and the sperm quality were assessed in normozoospermia (N) (42 men) and asthenoteratozoospermia (AT) (72 men) groups through sperm chromatin dispersion (SCD), sperm chromatin structure assay (SCSA), and chromatin maturation index (CMI).
resultsThe results showed the distribution of FN protein on the equatorial region of the human sperm surface. In addition, the FN levels were found to have a significant difference between the two groups with 24.64±9.08% in N and 16.90±7.27% in AT (p≤0.0001). In addition, FN level negatively correlated with SCD (p≤0.0001), SCSA (p≤0.0001), and CMI (p≤0.001). Threshold values of FN level and DNA fragmentation index (DFI) percentage were 16 and 30 and were identified as cut-off values to determine the N group with a specificity of 83.3% and 81.0% and a sensitivity of 16.8% and 19.0%, respectively. The specificity and sensitivity of FN-DFI were 91.2% and 8.8%, respectively.
conclusionIt appears that FN can be used for the selection of sperm with suitable quality, although future studies are recommended.
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Registered trials
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