ArticleMolecular microbiology2019
Systematic analysis of Type I-E Escherichia coli CRISPR-Cas PAM sequences ability to promote interference and primed adaptation.
Article in Molecular microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 35 citations in OpenAlex.
- TheEcoSal Plus · 2025Review
- Continuous targeted hypermutation with a tunable mutation window.Nature communications · 2025Article
- Harnessing the Streptomyces-originating type I-E CRISPR/Cas system for efficient genome editing in Streptomyces.Science China. Life sciences · 2025Article
- Article
- Structure and genome editing of type I-B CRISPR-Cas.Nature communications · 2024Article
- CRISPR-Cas adaptation in Escherichia coli.Bioscience reports · 2023Review
- Bio-informatic analysis of CRISPR protospacer adjacent motifs (PAMs) in T4 genome.BMC genomic data · 2022Article
- Function and Application of the CRISPR-Cas System in the Plant PathogenApplied and environmental microbiology · 2022Article
- Persistence of plasmids targeted by CRISPR interference in bacterial populations.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Single cell variability of CRISPR-Cas interference and adaptation.Molecular systems biology · 2022Article
- PAM-repeat associations and spacer selection preferences in single and co-occurring CRISPR-Cas systems.Genome biology · 2021Article
- Prespacers formed during primed adaptation associate with the Cas1-Cas2 adaptation complex and the Cas3 interference nuclease-helicase.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- The type I-E CRISPR-Cas system influences the acquisition ofEmerging microbes & infections · 2020Article
- Identification of Spacer and Protospacer Sequence Requirements in the Vibrio cholerae Type I-E CRISPR/Cas System.mSphere · 2020Article
- It is unclear how important CRISPR-Cas systems are for protecting natural populations of bacteria against infections by mobile genetic elements.Proceedings of the National Academy of Sciences of the United States of America · 2020Review
- Detection of CRISPR adaptation.Biochemical Society transactions · 2020Review
- Fidelity of prespacer capture and processing is governed by the PAM-mediated interactions of Cas1-2 adaptation complex in CRISPR-Cas type I-E system.The Journal of biological chemistry · 2019Article
- Cas9 Cleavage of Viral Genomes Primes the Acquisition of New Immunological Memories.Cell host & microbe · 2019Article
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Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
Abstract
CRISPR interference occurs when a protospacer recognized by the CRISPR RNA is destroyed by Cas effectors. In Type I CRISPR-Cas systems, protospacer recognition can lead to «primed adaptation» - acquisition of new spacers from in cis located sequences. Type I CRISPR-Cas systems require the presence of a trinucleotide protospacer adjacent motif (PAM) for efficient interference. Here, we investigated the ability of each of 64 possible trinucleotides located at the PAM position to induce CRISPR interference and primed adaptation by the Escherichia coli Type I-E CRISPR-Cas system. We observed clear separation of PAM variants into three groups: those unable to cause interference, those that support rapid interference and those that lead to reduced interference that occurs over extended periods of time. PAM variants unable to support interference also did not support primed adaptation; those that supported rapid interference led to no or low levels of adaptation, while those that caused attenuated levels of interference consistently led to highest levels of adaptation. The results suggest that primed adaptation is fueled by the products of CRISPR interference. Extended over time interference with targets containing «attenuated» PAM variants provides a continuous source of new spacers leading to high overall level of spacer acquisition.
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