Evidence map›Paper›PMID 30874553›Full record

ArticleNature communications2019

Automating multimodal microscopy with NanoJ-Fluidics.

Pedro Almada, Pedro M Pereira, Siân Culley, Ghislaine Caillol, Fanny Boroni-Rueda, Christina L Dix, Guillaume Charras, Buzz Baum, Romain F Laine, Christophe Leterrier and 1 more

Abstract read
In one paragraph

Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.

0numbers the graph read from it
0cells of the map it votes in
51citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

51 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Article
  6. Article
  7. Review
  8. Time-deterministic cryo-optical microscopy.Light, science & applications · 2025
    Article
  9. Review
  10. Article
  11. Article
  12. Article
  13. Live-cell imaging powered by computation.Nature reviews. Molecular cell biology · 2024
    Review
  14. Article
  15. Article
  16. Article
  17. Live-cell imaging in the deep learning era.Current opinion in cell biology · 2023
    Review
  18. Article
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Pedro AlmadaMRC-Laboratory for Molecular Cell Biology, University College London, London, WC1E 6BT, UK.
Pedro M PereiraMRC-Laboratory for Molecular Cell Biology, University College London, London, WC1E 6BT, UK.ORCID http://orcid.org/0000-0002-1426-9540
Siân CulleyMRC-Laboratory for Molecular Cell Biology, University College London, London, WC1E 6BT, UK.ORCID http://orcid.org/0000-0003-2112-0143
Ghislaine CaillolAix Marseille Université, CNRS, INP UMR7051, NeuroCyto, Marseille, 13015, France.
Fanny Boroni-RuedaAix Marseille Université, CNRS, INP UMR7051, NeuroCyto, Marseille, 13015, France.
Christina L DixMRC-Laboratory for Molecular Cell Biology, University College London, London, WC1E 6BT, UK.
Guillaume CharrasLondon Centre for Nanotechnology, London, WC1H 0AH, UK.
Buzz BaumMRC-Laboratory for Molecular Cell Biology, University College London, London, WC1E 6BT, UK.
Romain F LaineMRC-Laboratory for Molecular Cell Biology, University College London, London, WC1E 6BT, UK. r.laine@ucl.ac.uk.
Christophe LeterrierAix Marseille Université, CNRS, INP UMR7051, NeuroCyto, Marseille, 13015, France. christophe.leterrier@univ-amu.fr.ORCID http://orcid.org/0000-0002-2957-2032
Ricardo HenriquesMRC-Laboratory for Molecular Cell Biology, University College London, London, WC1E 6BT, UK. r.henriques@ucl.ac.uk.ORCID http://orcid.org/0000-0002-2043-5234

Funding

Biotechnology and Biological Sciences Research Council BB/P001440/1Cancer Research UK 17343Wellcome Trust
6 · The paper itself

Abstract

Combining and multiplexing microscopy approaches is crucial to understand cellular events, but requires elaborate workflows. Here, we present a robust, open-source approach for treating, labelling and imaging live or fixed cells in automated sequences. NanoJ-Fluidics is based on low-cost Lego hardware controlled by ImageJ-based software, making high-content, multimodal imaging easy to implement on any microscope with high reproducibility. We demonstrate its capacity on event-driven, super-resolved live-to-fixed and multiplexed STORM/DNA-PAINT experiments.

Identifiers

PMID30874553
PMCPMC6420627

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.