ArticleNature communications2019
Photoregulated fluxional fluorophores for live-cell super-resolution microscopy with no apparent photobleaching.
Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The trial behind it
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Who cites it
25 citing papers in PubMed.
- A Photoswitchable HaloTag for Spatiotemporal Control of Fluorescence in Living Cells.Angewandte Chemie (International ed. in English) · 2025Article
- Advancing Single-Molecule Biophysics: Next-Generation Organic Fluorophores with Tailored Labeling Strategies.Chemical & biomedical imaging · 2025Review
- Advancing Multicolor Super-Resolution Volume Imaging: Illuminating Complex Cellular Dynamics.JACS Au · 2025Review
- Photoswitchable Fluorescent Hydrazone for Super-Resolution Cell Membrane Imaging.Journal of the American Chemical Society · 2025Article
- Coumarin as a general switching auxiliary to prepare photochromic and spontaneously blinking fluorophores.bioRxiv : the preprint server for biology · 2024Article
- An open and shut case? Chemistry to control xanthene dyes.Trends in chemistry · 2024Article
- Light-Deactivated Fluorescent Probes (FLASH-Off) for Multiplexed Imaging.Journal of the American Chemical Society · 2023Article
- Single-Molecule Peptide Identification Using Fluorescence Blinking Fingerprints.Journal of the American Chemical Society · 2023Article
- Leveraging Baird aromaticity for advancement of bioimaging applications.Journal of physical organic chemistry · 2023Article
- Long-Term, Single-Molecule Imaging of Proteins in Live Cells with Photoregulated Fluxional Fluorophores.Chemistry (Weinheim an der Bergstrasse, Germany) · 2022Article
- resPAINT: Accelerating Volumetric Super-Resolution Localisation Microscopy by Active Control of Probe Emission.Angewandte Chemie (International ed. in English) · 2022Article
- resPAINT: Accelerating Volumetric Super-Resolution Localisation Microscopy by Active Control of Probe Emission.Angewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2022Article
- Detailed Imaging of Mitochondrial Transport and Precise Manipulation of Mitochondrial Function with Genetically Encoded Photosensitizers in Adult Drosophila Neurons.Methods in molecular biology (Clifton, N.J.) · 2022Article
- High-Resolution Imaging of Mitochondria and Mitochondrial Nucleoids in Differentiated SH-SY5Y Cells.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Unsupervised selection of optimal single-molecule time series idealization criterion.Biophysical journal · 2021Article
- A Continuous Add-On Probe Reveals the Nonlinear Enlargement of Mitochondria in Light-Activated Oncosis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Extremely Bright, Near-IR Emitting Spontaneously Blinking Fluorophores Enable Ratiometric Multicolor Nanoscopy in Live Cells.ACS central science · 2021Article
- Chemical Analysis of Single Cells and Organelles.Analytical chemistry · 2021Review
- Recent Progress in Small Spirocyclic, Xanthene-Based Fluorescent Probes.Molecules (Basel, Switzerland) · 2020Review
- Three-Dimensional Single-Molecule Localization Microscopy in Whole-Cell and Tissue Specimens.Annual review of biomedical engineering · 2020Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Photoswitchable molecules have multiple applications in the physical and life sciences because their properties can be modulated with light. Fluxional molecules, which undergo rapid degenerate rearrangements in the electronic ground state, also exhibit switching behavior. The stochastic nature of fluxional switching, however, has hampered its application in the development of functional molecules and materials. Here we combine photoswitching and fluxionality to develop a fluorophore that enables very long (>30 min) time-lapse single-molecule localization microscopy in living cells with minimal phototoxicity and no apparent photobleaching. These long time-lapse experiments allow us to track intracellular organelles with unprecedented spatiotemporal resolution, revealing new information of the three-dimensional compartmentalization of synaptic vesicle trafficking in live human neurons.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.