ArticleJournal of clinical and translational research2015
Layer-by-layer heparinization of decellularized liver matrices to reduce thrombogenicity of tissue engineered grafts.
Article in Journal of clinical and translational research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 39 citations in OpenAlex.
- Decellularization of Human Digits: A Step Towards Off-the-Shelf Composite Allograft Transplantation.Bioengineering (Basel, Switzerland) · 2025Article
- Decellularized Liver Matrices for Expanding the Donor Pool-An Evaluation of Existing Protocols and Future Trends.Biomolecules · 2025Review
- Heparin Immobilization Enhances Hemocompatibility, Re-Endothelization, and Angiogenesis of Decellularized Liver Scaffolds.International journal of molecular sciences · 2024Article
- Two Decades of Advances and Limitations in Organ Recellularization.Current issues in molecular biology · 2024Review
- Review
- Advances in lung bioengineering: Where we are, where we need to go, and how to get there.Frontiers in transplantation · 2023Review
- In-Vitro Endothelialization Assessment of Heparinized Bovine Pericardial Scaffold for Cardiovascular Application.Polymers · 2022Article
- Review
- Recent Advances in Liver Engineering With Decellularized Scaffold.Frontiers in bioengineering and biotechnology · 2022Review
- Biomaterial-based cell delivery strategies to promote liver regeneration.Biomaterials research · 2021Review
- Decellularized Whole-Organ Pre-vascularization: A Novel Approach for Organogenesis.Frontiers in bioengineering and biotechnology · 2021Article
- Engineering liver microtissues for disease modeling and regenerative medicine.Advanced functional materials · 2020Article
- A Hepatic Scaffold from Decellularized Liver Tissue: Food for Thought.Biomolecules · 2019Review
- A Novel Surgical Technique As a Foundation for In Vivo Partial Liver Engineering in Rat.Journal of visualized experiments : JoVE · 2018Article
- Improving functional re-endothelialization of acellular liver scaffold using REDV cell-binding domain.Acta biomaterialia · 2018Article
- Article
- Liver tissue engineering: From implantable tissue to whole organ engineering.Hepatology communications · 2018Review
- Discarded Livers Find a New Life: Engineered Liver Grafts Using Hepatocytes Recovered From Marginal Livers.Artificial organs · 2017Article
- Targeting Heparin to Collagen within Extracellular Matrix Significantly Reduces Thrombogenicity and Improves Endothelialization of Decellularized Tissues.Biomacromolecules · 2016Article
- Editor's inaugural issue foreword: perspectives on translational and clinical research.Journal of clinical and translational research · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundTissue-engineered liver grafts may offer a viable alternative to orthotopic liver transplantation and help overcome the donor organ shortage. Decellularized liver matrices (DLM) have a preserved vasculature and sustain hepatocellular function in culture, but graft survival after transplantation remains limited due to thrombogenicity of the matrix.
aimTo evaluate the effect of heparin immobilization on DLM thrombogenicity.
methodsHeparin was immobilized on DLMs by means of layer-by-layer deposition. Grafts with 4 or 8 bilayers and 2 or 4 g/L of heparin were recellularized with primary rat hepatocytes and maintained in culture for 5 days. Hemocompatibility of the graft was assessed by ex vivo diluted whole-blood perfusion and heterotopic transplantation.
resultsHeparin was deposited throughout the matrix and the heparin content in the graft was higher with increasing number of bilayers and concentration of heparin. Recellularization and in vitro albumin and urea production were unaffected by heparinization. Resistance to blood flow during ex vivo perfusion was lower with increased heparinization and, macroscopically, no clots were visible in grafts with 8 bilayers. Following transplantation, flow through the graft was limited in all groups. Histological evidence of thrombosis was lower in heparinized DLMs, but transplantation of DLM grafts was not improved.
conclusionsLayer-by-layer deposition of heparin on a DLM is an effective method of immobilizing heparin throughout the graft and does not impede recellularization or hepatocellular function in vitro. Thrombogenicity during ex vivo blood perfusion was reduced in heparinized grafts and optimal with 8 bilayers, but transplantation remained unsuccessful with this method. RELEVANCE FOR PATIENTS: Tissue engineered liver grafts may offer a viable solution to dramatic shortages in donor organs.
Indexed as
Identifiers
30873444PMC4607069W2110353731What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.