ArticleSynthetic biology (Oxford, England)2019
Cell-free biosynthesis of limonene using enzyme-enriched
Article in Synthetic biology (Oxford, England), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 86 citations in OpenAlex.
- Timed batch inputs unlock substantially higher yields for enzymatic cascades.Nature chemistry · 2026Article
- The Mevalonate Pathway: Innovations, Applications, and Challenges in Biotechnology with Emphasis on Fungal Biology.Journal of fungi (Basel, Switzerland) · 2026Review
- Machine learning-driving optimization and spatial assembly of a cell-free system for high-yield liquiritigenin production.Advanced biotechnology · 2026Article
- Design-driven optimization of low-cost reagent formulations for reproducible and high-yielding cell-free gene expression.Nature communications · 2026Article
- A Simple and Versatile Cell-Free Expression Method for Producing Secondary Metabolites.ACS synthetic biology · 2026Article
- A Rapid and Modular Nanobody Assay for Plug-and-Play Antigen Detection.bioRxiv : the preprint server for biology · 2025Article
- Cell-Free Gene Expression: Methods and Applications.Chemical reviews · 2025Review
- Applications of the wheat germ cell-free protein synthesis system in plant biochemical studies.Plant biotechnology (Tokyo, Japan) · 2024Article
- Exploring Emergent Properties in Enzymatic Reaction Networks: Design and Control of Dynamic Functional Systems.Chemical reviews · 2024Review
- Engineering the thermal stability of a polyphosphate kinase by ancestral sequence reconstruction to expand the temperature boundary for an industrially applicable ATP regeneration system.Applied and environmental microbiology · 2024Article
- When synthetic biology meets medicine.Life medicine · 2024Review
- Investigating ethanol production using the Zymomonas mobilis crude extract.Scientific reports · 2023Article
- Rewiring cell-free metabolic flux inSynthetic biology (Oxford, England) · 2023Article
- Cell-Free Protein Synthesis of Particulate Methane Monooxygenase into Nanodiscs.ACS synthetic biology · 2022Article
- Monoterpenoid biosynthesis by engineered microbes.Journal of industrial microbiology & biotechnology · 2021Review
- A roadmap for metagenomic enzyme discovery.Natural product reports · 2021Review
- Optimization of E. Coli Tip-Sonication for High-Yield Cell-Free Extract using Finite Element Modeling.AIChE journal. American Institute of Chemical Engineers · 2021Article
- A Modular In Vitro Platform for the Production of Terpenes and Polyketides from COAngewandte Chemie (International ed. in English) · 2021Article
- A lysate proteome engineering strategy for enhancing cell-free metabolite production.Metabolic engineering communications · 2021Article
- Genetic and metabolic engineering challenges of C1-gas fermenting acetogenic chassis organisms.FEMS microbiology reviews · 2021Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Isoprenoids are an attractive class of metabolites for enzymatic synthesis from renewable substrates. However, metabolic engineering of microorganisms for monoterpenoid production is limited by the need for time-consuming, and often non-intuitive, combinatorial tuning of biosynthetic pathway variations to meet design criteria. Towards alleviating this limitation, the goal of this work was to build a modular, cell-free platform for construction and testing of monoterpenoid pathways, using the fragrance and flavoring molecule limonene as a model. In this platform, multiple
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.