ArticleScientific reports2019
Cellulose fibrils formation and organisation of cytoskeleton during encystment are essential for Acanthamoeba cyst wall architecture.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it, 57 citations in OpenAlex.
- Prevalence of free-living amoebae in swimming pools and recreational waters, a systematic review and meta-analysis.Parasitology research · 2022Pooled it
- True cyst formation underlies persistence and drug tolerance in Tritrichomonas foetus.Nature communications · 2026Article
- New Viability Assays for Acanthamoeba castellanii Trophozoites and Cysts Allow Rapid Assessment of Drug Activity.The Journal of infectious diseases · 2026Article
- Signal peptide variation in cyst lectins as a potential marker for pathogenic Acanthamoeba spp.Parasites & vectors · 2026Article
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- Demethoxycurcumin exhibits amoebicidal activity againstCurrent research in parasitology & vector-borne diseases · 2026Article
- Emerging Ocular Pathogen Resistance and Clinically Used Solutions: A Problem That Is More than Meets the Eye.Pharmaceuticals (Basel, Switzerland) · 2025Review
- Encystment and Excystment Processes inPathogens (Basel, Switzerland) · 2025Review
- Agar dehydration: a simple method for long-term storage of Acanthamoeba spp. collection at room temperature.Parasitology research · 2024Article
- Glucose metabolism in the pathogenic free-living amoebae: Tempting targets for treatment development.Chemical biology & drug design · 2024Review
- Plants of the family Lamiaceae as a source of therapeutic agents against Acanthamoeba infections.Memorias do Instituto Oswaldo Cruz · 2024Review
- Novel Insights into the Wattle and Daub Model ofPathogens (Basel, Switzerland) · 2023Article
- Encystation and Stress Responses under the Control of Ubiquitin-like Proteins in Pathogenic Amoebae.Microorganisms · 2023Review
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- Biological characteristics and pathogenicity ofFrontiers in microbiology · 2023Review
- Characterization of Glucokinases from Pathogenic Free-Living Amoebae.Antimicrobial agents and chemotherapy · 2022Article
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- Curcumin effect onPeerJ · 2022Article
- Stimulation ofFrontiers in cell and developmental biology · 2022Article
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Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acanthamoebae success as human pathogens is largely due to the highly resistant cysts which represent a crucial problem in treatment of Acanthamoeba infections. Hence, the study of cyst wall composition and encystment play an important role in finding new therapeutic strategies. For the first time, we detected high activity of cytoskeletal elements - microtubular networks and filamentous actin, in late phases of encystment. Cellulose fibrils - the main components of endocyst were demonstrated in inter-cystic space, and finally in the ectocyst, hereby proving the presence of cellulose in both layers of the cyst wall. We detected clustering of intramembranous particles (IMPs) and their density alterations in cytoplasmic membrane during encystment. We propose a hypothesis that in the phase of endocyst formation, the IMP clusters represent cellulose microfibril terminal complexes involved in cellulose synthesis that after cyst wall completion are reduced. Cyst wall impermeability, due largely to a complex polysaccharide (glycans, mainly cellulose) has been shown to be responsible for Acanthamoeba biocide resistance and cellulose biosynthesis pathway is suggested to be a potential target in treatment of Acanthamoeba infections. Disruption of this pathway would affect the synthesis of cyst wall and reduce considerably the resistance to chemotherapeutic agents.
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