ArticleScientific reports2019
Shotgun Proteomics of Human Dentin with Different Prefractionation Methods.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 50 citations in OpenAlex.
- Therapeutic Irrigant Procedures for Treating Apical Periodontitis (TIPTAP): A triple-blinded parallel-group randomized controlled phase I/II trial.International endodontic journal · 2025Trial
- Stage-Specific Proteomic Profiles in Dental Caries.Journal of dental research · 2026Article
- Inflammation and response to bacterial infection as potential drivers of equine odontoclastic tooth resorption and hypercementosis: A proteomics insight.Equine veterinary journal · 2025Article
- Enhancing Mass spectrometry-based tumor immunopeptide identification: machine learning filter leveraging HLA binding affinity, aliphatic index and retention time deviation.Computational and structural biotechnology journal · 2024Article
- Public RNA-seq data-based identification and functional analyses reveal that MXRA5 retains proliferative and migratory abilities of dental pulp stem cells.Scientific reports · 2023Article
- Novel Functional Peptide for Next-Generation Vital Pulp Therapy.Journal of dental research · 2023Article
- Bioactivity of Dental Restorative Materials: FDI Policy Statement.International dental journal · 2023Review
- A Compilation of Study Models for Dental Pulp Regeneration.International journal of molecular sciences · 2022Review
- Paleoproteomics.Chemical reviews · 2022Review
- Isolation of Endogenous TGF-β1 from Root Canals for Pulp Tissue Engineering: A Translational Study.Biology · 2022Article
- Complementing the pulp proteome via sampling with a picosecond infrared laser (PIRL).Clinical oral investigations · 2021Article
- Proteomic and genomic analysis of acid dentin lysate with focus on TGF-β signaling.Scientific reports · 2021Article
- Article
- Inflammatory Response Mechanisms of the Dentine-Pulp Complex and the Periapical Tissues.International journal of molecular sciences · 2021Review
- Large-scale production, purification, and function of a tumor multi-epitope vaccine: Peptibody with bFGF/VEGFA.Engineering in life sciences · 2020Article
- Mapping and Identification of Native Proteins of Developing Teeth in Mouse Mandibles.Analytical chemistry · 2020Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human dentin is not only a composite material of a collagenous matrix and mineral to provide strength and elasticity to teeth, but also a precious reservoir full of bioactive proteins. They are released after demineralization caused by bacterial acids in carious lesions, by decalcifying irrigants or dental materials and they modulate tissue responses in the underlying dental pulp. This work describes a first-time analysis of the proteome of human dentin using a shotgun proteomic approach that combines three different protein fractionation methods. Dentin matrix proteins were extracted by EDTA and separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), OFFGEL isoelectric focusing (IEF) or strong cation exchange chromatography (SCX). Liquid chromatography tandem mass spectrometry (LC-MS/MS) identified 813 human proteins with high confidence, however, isoelectric focusing turned out to be the most beneficial prefractionation method. All Proteins were categorized based on the PANTHER system and representation analysis revealed 31 classes and subclasses to be overrepresented. The acquired knowledge provides a comprehensive insight into the number of proteins in human dentin as well as their physiological and pathological functions. Thus, the data presented paves the way to the analysis of specific functions of dentin matrix proteins in vivo and their potential in tissue engineering approaches to regenerate dental pulp.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.