ArticleScientific reports2019
Transcriptomic and lipidomic analysis of an EPA-containing Nannochloropsis sp. PJ12 in response to nitrogen deprivation.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- The life cycle of fatty acids in oleaginous stramenopiles: from synthesis to maturation and beyond.BMC plant biology · 2026Review
- Stimulating EPA Biosynthesis inJournal of microbiology and biotechnology · 2025Article
- Review
- Reconstruction of Long-Chain Polyunsaturated Acid Synthesis Pathways in Marine Red MicroalgaMarine drugs · 2024Article
- Pilot Lipidomics Study of Copepods: Investigation of Potential Lipid-Based Biomarkers for the Early Detection and Quantification of the Biological Effects of Climate Change on the Oceanic Food Chain.Life (Basel, Switzerland) · 2023Article
- Biodiesel Production from the Marine AlgaPlants (Basel, Switzerland) · 2023Article
- Lipidomic and transcriptomic profiles of glycerophospholipid metabolism during Hemerocallis citrina Baroni flowering.BMC plant biology · 2023Article
- Culture Conditions Affect Antioxidant Production, Metabolism and Related Biomarkers of the MicroalgaeAntioxidants (Basel, Switzerland) · 2022Article
- Retrograde and anterograde signaling in the crosstalk between chloroplast and nucleus.Frontiers in plant science · 2022Review
- Comparative Proteomics Reveals Evidence of Enhanced EPA Trafficking in a Mutant Strain ofFrontiers in bioengineering and biotechnology · 2022Article
- Lipid turnover and SQUAMOSA promoter-binding proteins mediate variation in fatty acid desaturation under early nitrogen deprivation revealed by lipidomic and transcriptomic analyses inFrontiers in plant science · 2022Article
- Physiological and transcriptome analysis elucidates the metabolic mechanism of versatile Porphyridium purpureum under nitrogen deprivation for exopolysaccharides accumulation.Bioresources and bioprocessing · 2021Article
- Integrated Lipidomic and Transcriptomic Analysis Reveals Lipid Metabolism in Foxtail Millet (Frontiers in genetics · 2021Article
- Lipid Droplets in Unicellular Photosynthetic Stramenopiles.Frontiers in plant science · 2021Review
- Nutritional evaluation and human health-promoting potential of compounds biosynthesized by native microalgae from the Peruvian Amazon.World journal of microbiology & biotechnology · 2020Article
- Surviving Starvation: Proteomic and Lipidomic Profiling of Nutrient Deprivation in the Smallest Known Free-Living Eukaryote.Metabolites · 2020Article
- Genetic Transformation ofInternational journal of molecular sciences · 2020Article
- Improved lipid productivity inBiotechnology for biofuels · 2020Article
- Transcriptomic Analysis of the Cold-Pretreated Larimichthys crocea Showing Enhanced Growth Fitness in Cold Water.Marine biotechnology (New York, N.Y.) · 2019Article
- Phosphorus-Induced Lipid Class Alteration Revealed by Lipidomic and Transcriptomic Profiling in Oleaginous MicroalgaMarine drugs · 2019Article
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3 authors.
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Abstract
To understand genes involved in neutral lipid accumulation upon nitrogen deprivation (ND) in a novel isolate of Nannochloropsis sp. PJ12, we performed comparative transcriptomic and lipidomic analyses of cells under ND and NR (nitrogen replete) conditions. Transcriptomic profiling indicated that, while enzymes involved in TCA cycle in PJ12 under ND condition were upregulated compared to that under NR condition, those involved in Calvin cycle and glycolysis under ND condition were downregulated. Furthermore, we showed that enzymes involved in fatty acid synthesis and glycerolipid synthesis were downregulated but not β-oxidation. Lipidomic profiling indicated that, while the level of neutral lipids in ND cells was increased compared to that of NR cells, level of photosynthetic membrane-lipids DGDG and PG was decreased. Taken together, our analysis indicated that TAG accumulation is attributed to the modification of membrane lipids derived primarily from "prokaryotic" pathway and secondarily from "eukaryotic" pathway based on the 16:X or 18:X fatty acid at the sn2 position of the glycerol backbone. We propose that two-phase (NR-ND) growth is ideal for biomass and biofuel production because ND reduces cell growth rate due to the loss of photosynthetic membrane and decreased quantum yield.
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