ArticleScientific reports2019
Human cytomegalovirus hijacks the autophagic machinery and LC3 homologs in order to optimize cytoplasmic envelopment of mature infectious particles.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 67 citations in OpenAlex.
- Glutamine supports human cytomegalovirus induced lipidome remodeling through reductive carboxylation.PLoS pathogens · 2026Article
- Autophagy machinery as exploited by viruses.Autophagy reports · 2025Article
- GABARAP proteins regulate the packaging of HIV-1 genomic RNA into virions.EMBO reports · 2025Article
- Molecular features of congenital cytomegalovirus infection in neonatal mouse brain at single-cell resolution.Acta neuropathologica communications · 2025Article
- The Cell Biologist Potential of Cytomegalovirus to Solve Biogenesis and Maintenance of the Membrane Recycling System.Biomedicines · 2025Article
- The Autophagy ReceptorViruses · 2024Article
- Viruses and autophagy: bend, but don't break.Nature reviews. Microbiology · 2024Review
- Intermittent cytomegalovirus infection alters neurobiological metabolism and induces cognitive deficits in mice.Brain, behavior, and immunity · 2024Article
- SNX27:Retromer:ESCPE-1-mediated early endosomal tubulation impacts cytomegalovirus replication.Frontiers in cellular and infection microbiology · 2024Article
- Rab10-associated tubulation as an early marker for biogenesis of the assembly compartment in cytomegalovirus-infected cells.Frontiers in cell and developmental biology · 2024Article
- The autophagy machinery interacts with EBV capsids during viral envelope release.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Article
- Secretory autophagy promotes RAB37-mediated insulin secretion under glucose stimulation bothAutophagy · 2023Article
- The role of autophagy in viral infections.Journal of biomedical science · 2023Review
- Cytomegaloviruses reorganize endomembrane system to intersect endosomal and amphisome-like egress pathway.Frontiers in cell and developmental biology · 2023Article
- Subviral Dense Bodies of Human Cytomegalovirus Induce an Antiviral Type I Interferon Response.Cells · 2022Article
- Article
- Host Cell Signatures of the Envelopment Site within Beta-Herpes Virions.International journal of molecular sciences · 2022Review
- Intermittent bulk release of human cytomegalovirus.PLoS pathogens · 2022Article
- Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During its life cycle, Human cytomegalovirus (HCMV) tightly modulates autophagy, a vesicular pathway allowing degradation and recycling of cellular components. To study the interplay between autophagy and the viral life cycle, we established various autophagy-deficient human fibroblastic cell lines. By knocking down the expression or activity of five autophagy-related proteins, we confirmed the proviral function that the autophagic machinery exerts on HCMV production. Using 3D reconstruction from confocal microscopy and electron microscopy, we demonstrated that lipidated LC3-positive vesicles accumulated at the viral assembly compartment (vAC). The vAC is a juxtanuclear ring-shaped structure containing several organelles and membranes, where assembly and final envelopment of HCMV particles occur. Two LC3 homologs, GABARAPL1 and GATE16, also accumulated during HCMV infection and were associated with the vAC, in proximity with fragmented Golgi stacks. Additionally, we observed the formation of a pre-assembly compartment (PrAC) in infected cells, which consists of a juxtanuclear structure containing both fragmented Golgi and LC3-positive vesicles. Finally, we showed that highly purified extracellular viral particles were associated with various autophagy proteins. Our results thus suggest that autophagy machinery participates to the final cytoplasmic envelopment of HCMV viral particles into the vAC and that autophagy-related proteins can be spotted in the virions.
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