ArticleScientific reports2019
High-throughput screening and classification of chemicals and their effects on neuronal gene expression using RASL-seq.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Article
- Progress in toxicogenomics to protect human health.Nature reviews. Genetics · 2025Review
- Co-detection of azoxystrobin and thiabendazole fungicides in mold and mildew resistant wallboards and in children.Heliyon · 2024Article
- TORNADO-seq: A Protocol for High-Throughput Targeted RNA-seq-Based Drug Screening in Organoids.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Article
- Multi-omic profiling reveals the ataxia protein sacsin is required for integrin trafficking and synaptic organization.Cell reports · 2022Article
- ToxCast chemical library Wnt screen identifies diethanolamine as an activator of neural progenitor proliferation.FASEB bioAdvances · 2022Article
- More Pieces of the Puzzle: New Insights into Azoxystrobin Exposures and Neurotoxicity.Environmental health perspectives · 2022Article
- Detection of Azoxystrobin Fungicide and Metabolite Azoxystrobin-Acid in Pregnant Women and Children, Estimation of Daily Intake, and Evaluation of Placental and Lactational Transfer in Mice.Environmental health perspectives · 2022Article
- Advances and Trends in Omics Technology Development.Frontiers in medicine · 2022Review
- Azoxystrobin exposure impairs meiotic maturation by disturbing spindle formation in mouse oocytes.Frontiers in cell and developmental biology · 2022Article
- Mapping the cellular response to electron transport chain inhibitors reveals selective signaling networks triggered by mitochondrial perturbation.Archives of toxicology · 2022Article
- In utero pyrethroid pesticide exposure in relation to autism spectrum disorder (ASD) and other neurodevelopmental outcomes at 3 years in the MARBLES longitudinal cohort.Environmental research · 2021Article
- Discovery of small molecules that normalize the transcriptome and enhance cysteine cathepsin activity in progranulin-deficient microglia.Scientific reports · 2020Article
- Phenotypic Profiling of Reference Chemicals across Biologically Diverse Cell Types Using the Cell Painting Assay.SLAS discovery : advancing life sciences R & D · 2020Article
- Comparison of Normalization Methods for Analysis of TempO-Seq Targeted RNA Sequencing Data.Frontiers in genetics · 2020Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
We previously used RNA-seq to identify chemicals whose effects on neuronal gene expression mimicked transcriptional signatures of autism, aging, and neurodegeneration. However, this approach was costly and time consuming, which limited our study to testing a single chemical concentration on mixed sex cortical neuron cultures. Here, we adapted a targeted transcriptomic method (RASL-seq, similar to TempO-seq) to interrogate changes in expression of a set of 56 signature genes in response to a library of 350 chemicals and chemical mixtures at four concentrations in male and female mouse neuronal cultures. This enabled us to replicate and expand our previous classifications, and show that transcriptional responses were largely equivalent between sexes. Overall, we found that RASL-seq can be used to accelerate the pace at which chemicals and mixtures that transcriptionally mimic autism and other neuropsychiatric diseases can be identified, and provides a cost-effective way to quantify gene expression with a panel of marker genes.
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