ArticlePlant physiology2019
Tandem Fluorescent Protein Timers for Noninvasive Relative Protein Lifetime Measurement in Plants.
Article in Plant physiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- A suite of fluorescent timers for visualizing protein spatiotemporal dynamics in plants.Science advances · 2026Article
- An Unusual Component of the Multistep Phosphorelay from Tea Plant (International journal of molecular sciences · 2026Article
- Unlocking the black box: multimodal imaging and quantitative analysis of plant vesicular trafficking.Advanced biotechnology · 2026Review
- Article
- Evidence for Functionality of Transmembrane Domains of Predicted Non-Canonical Plant Phosphotransmitters.Doklady. Biochemistry and biophysics · 2025Article
- An improved toolkit of gateway- and gibson assembly-compatible vectors for protoplast transfection and agrobacterium-mediated plant transformation.BMC research notes · 2025Article
- A dynamic ubiquitination balance of cell proliferation and endoreduplication regulators determines plant organ size.Science advances · 2024Article
- Relative Protein Lifetime Measurement in Plants Using Tandem Fluorescent Protein Timers.Methods in molecular biology (Clifton, N.J.) · 2023Article
- HYPK promotes the activity of theScience advances · 2022Article
- Cotranslational N-degron masking by acetylation promotes proteome stability in plants.Nature communications · 2022Article
- Indirect Methods To Measure Unfolded Proteins In Living Cells Using Fluorescent Proteins.Methods in molecular biology (Clifton, N.J.) · 2022Article
- High-Throughput Analysis of Protein Turnover with Tandem Fluorescent Protein Timers.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Current and future advances in fluorescence-based visualization of plant cell wall components and cell wall biosynthetic machineries.Biotechnology for biofuels · 2021Review
- A Yeast-Based Functional Assay to Study Plant N-Degron - N-Recognin Interactions.Frontiers in plant science · 2021Article
- Synthetic biology of hypoxia.The New phytologist · 2021Review
- Differential N-end Rule Degradation of RIN4/NOI Fragments Generated by the AvrRpt2 Effector Protease.Plant physiology · 2019Article
- Timing Is Everything: Tandem Fluorescent Timers Expand Our Understanding of Protein Longevity.Plant physiology · 2019Article
- Unresolved roles of Aux/IAA proteins in auxin responses.Physiologia plantarumReview
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7 authors.
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Abstract
Targeted protein degradation is an important and pervasive regulatory mechanism in plants, required for perception and response to the environment as well as developmental signaling. Despite the significance of this process, relatively few studies have assessed plant protein turnover in a quantitative fashion. Tandem fluorescent protein timers (tFTs) offer a powerful approach for the assessment of in vivo protein turnover in distinct subcellular compartments of single or multiple cells. A tFT is a fusion of two different fluorescent proteins with distinct fluorophore maturation kinetics, which enable protein age to be estimated from the ratio of fluorescence intensities of the two fluorescent proteins. Here, we used short-lived auxin signaling proteins and model N-end rule (N-recognin) pathway reporters to demonstrate the utility of tFTs for studying protein turnover in living plant cells of Arabidopsis (
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