Evidence map›Paper›PMID 30872394›Full record

ArticleGut2019

Crypt fusion as a homeostatic mechanism in the human colon.

Ann-Marie Baker, Calum Gabbutt, Marc J Williams, Biancastella Cereser, Noor Jawad, Manuel Rodriguez-Justo, Marnix Jansen, Chris P Barnes, Benjamin D Simons, Stuart Ac McDonald and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Gut, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Review
  5. Article
  6. Article
  7. Article
  8. Article
  9. Review
  10. Lineage tracing in human tissues.The Journal of pathology · 2022
    Review
  11. Review
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Review
  18. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Ann-Marie Baker *Centre for Tumour Biology, Barts Cancer Institute, Queen Mary University of London, London, UK.
Calum Gabbutt *Centre for Tumour Biology, Barts Cancer Institute, Queen Mary University of London, London, UK.
Marc J WilliamsCentre for Tumour Biology, Barts Cancer Institute, Queen Mary University of London, London, UK.ORCID 0000-0001-5524-4174
Biancastella CereserCentre for Tumour Biology, Barts Cancer Institute, Queen Mary University of London, London, UK.
Noor JawadCentre for Tumour Biology, Barts Cancer Institute, Queen Mary University of London, London, UK.
Manuel Rodriguez-JustoHistopathology, University College London, London, UK.
Marnix JansenHistopathology, University College London, London, UK.
Chris P BarnesDepartment of Cell and Developmental Biology, University College London, London, UK.ORCID 0000-0002-9459-1395
Benjamin D SimonsCavendish Laboratory, Department of Physics, University of Cambridge, Cambridge, UK.
Stuart Ac McDonaldCentre for Tumour Biology, Barts Cancer Institute, Queen Mary University of London, London, UK.
Trevor A GrahamCentre for Tumour Biology, Barts Cancer Institute, Queen Mary University of London, London, UK.ORCID 0000-0001-9582-1597
Nicholas A WrightCentre for Tumour Biology, Barts Cancer Institute, Queen Mary University of London, London, UK.

Funding

The Role of the Microbiome in Cancer Suppression and Susceptibility Across SpeciesU54CA217376 · NCI · ARIZONA STATE UNIVERSITY-TEMPE CAMPUS · PI MALEY, CARLO · 2018 to 2022
$8.6M
Biotechnology and Biological Sciences Research CouncilCancer Research UK 14895Cancer Research UK 19771Cancer Research UK 27145Cancer Research UK A14895Cancer Research UK A19771Medical Research Council G0901178Medical Research Council MC_PC_12009NCI NIH HHS U54 CA217376Wellcome Trust 098357Wellcome Trust 202778/Z/16/ZWellcome Trust 209409/Z/17/Z
6 · The paper itself

Abstract

objectiveThe crypt population in the human intestine is dynamic: crypts can divide to produce two new daughter crypts through a process termed crypt fission, but whether this is balanced by a second process to remove crypts, as recently shown in mouse models, is uncertain. We examined whether crypt fusion (the process of two neighbouring crypts fusing into a single daughter crypt) occurs in the human colon.

designWe used somatic alterations in the gene cytochrome c oxidase (CCO) as lineage tracing markers to assess the clonality of bifurcating colon crypts (n=309 bifurcating crypts from 13 patients). Mathematical modelling was used to determine whether the existence of crypt fusion can explain the experimental data, and how the process of fusion influences the rate of crypt fission.

resultsIn 55% (21/38) of bifurcating crypts in which clonality could be assessed, we observed perfect segregation of clonal lineages to the respective crypt arms. Mathematical modelling showed that this frequency of perfect segregation could not be explained by fission alone (p<10

conclusionsWe have provided the evidence that human colonic crypts undergo fusion, a potential homeostatic process to regulate total crypt number. The existence of crypt fusion in the human colon adds a new facet to our understanding of the highly dynamic and plastic phenotype of the colonic epithelium.

Indexed as

Aberrant Crypt FociAdultAgedCell Culture TechniquesCell FusionColonElectron Transport Complex IVFemaleHomeostasisHumansIntestinal MucosaMaleMiddle AgedModels, TheoreticalElectron Transport Complex IVcolon cryptcrypt fissioncrypt fusionevolutionary dynamicslineage tracingmathematical modelling

Identifiers

PMID30872394
PMCPMC6839731

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.