ArticleEpigenetics & chromatin2019
Targeted in vivo epigenome editing of H3K27me3.
Article in Epigenetics & chromatin, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Epigenetic editing: from concept to clinic.Nature reviews. Drug discovery · 2026Review
- A homozygous variant in HFM1 causes preimplantation embryo developmental arrest by disrupting zygotic genome activation.Human reproduction (Oxford, England) · 2026Article
- Epigenetic editing of marine medaka (Zoological research · 2026Article
- Programmable genome engineering and gene modifications for plant biodesign.Plant communications · 2025Review
- Epigenetics and individuality: from concepts to causality across timescales.Nature reviews. Genetics · 2025Review
- CRISPR/dCas9-targeted H3K27me3 demethylation at theiScience · 2025Article
- CRISPR-broad: combined design of multi-targeting gRNAs and broad, multiplex target finding.Scientific reports · 2023Article
- Incomplete erasure of histone marks during epigenetic reprogramming in medaka early development.Genome research · 2023Article
- Toward the Development of Epigenome Editing-Based Therapeutics: Potentials and Challenges.International journal of molecular sciences · 2023Review
- Targeted Manipulation of Histone Modification in Medaka Embryos.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Histone Marks-Dependent Effect on Alternative Splicing: New Perspectives for Targeted Splicing Modulation in Cancer?International journal of molecular sciences · 2022Review
- Epigenetic Editing in Prostate Cancer: Challenges and Opportunities.Epigenetics · 2022Review
- The Activation of Protamine 1 Using Epigenome Editing Decreases the Proliferation of Tumorigenic Cells.Frontiers in genome editing · 2022Article
- Hit-and-Run Epigenetic Editing for Vectors of Snail-Borne Parasitic Diseases.Frontiers in cell and developmental biology · 2022Review
- Deciphering Plant Chromatin Regulation via CRISPR/dCas9-Based Epigenome Engineering.Epigenomes · 2021Review
- Chromatin Manipulation and Editing: Challenges, New Technologies and Their Use in Plants.International journal of molecular sciences · 2021Review
- Applications of CRISPR technologies in transplantation.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2020Review
- Seq-ing Higher Ground: Functional Investigation of Adaptive Variation Associated With High-Altitude Adaptation.Frontiers in genetics · 2020Article
- Therapeutic applications of CRISPR/Cas9 in breast cancer and delivery potential of gold nanomaterials.NanobiomedicineArticle
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEpigenetic modifications have a central role in transcriptional regulation. While several studies using next-generation sequencing have revealed genome-wide associations between epigenetic modifications and transcriptional states, a direct causal relationship at specific genomic loci has not been fully demonstrated, due to a lack of technology for targeted manipulation of epigenetic modifications. Recently, epigenome editing techniques based on the CRISPR-Cas9 system have been reported to directly manipulate specific modifications at precise genomic regions. However, the number of editable modifications as well as studies applying these techniques in vivo is still limited.
resultsHere, we report direct modification of the epigenome in medaka (Japanese killifish, Oryzias latipes) embryos. Specifically, we developed a method to ectopically induce the repressive histone modification, H3K27me3 in a locus-specific manner, using a fusion construct of Oryzias latipes H3K27 methyltransferase Ezh2 (olEzh2) and dCas9 (dCas9-olEzh2). Co-injection of dCas9-olEzh2 mRNA with single guide RNAs (sgRNAs) into one-cell-stage embryos induced specific H3K27me3 accumulation at the targeted loci and induced downregulation of gene expression.
conclusionIn this study, we established the in vivo epigenome editing of H3K27me3 using medaka embryos. The locus-specific manipulation of the epigenome in living organisms will lead to a previously inaccessible understanding of the role of epigenetic modifications in development and disease.
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