Evidence map›Paper›PMID 30871557›Full record

ArticleJournal of translational medicine2019

Polyethylene glycol improves current methods for circulating extracellular vesicle-derived DNA isolation.

N García-Romero, R Madurga, G Rackov, I Palacín-Aliana, R Núñez-Torres, A Asensi-Puig, J Carrión-Navarro, S Esteban-Rubio, H Peinado, A González-Neira and 3 more

Abstract read
In one paragraph

Article in Journal of translational medicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.

0numbers the graph read from it
0cells of the map it votes in
69citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

69 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
  4. Review
  5. Review
  6. Article
  7. Review
  8. Review
  9. Article
  10. Review
  11. Review
  12. Article
  13. Review
  14. Article
  15. Exosome isolation based on polyethylene glycol (PEG): a review.Molecular and cellular biochemistry · 2025
    Review
  16. Article
  17. Article
  18. Review
  19. Exosome enrichment from human serum using polyethylene glycol precipitation.Journal of analytical science and technology · 2025
    Article
  20. Review

9 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

N García-RomeroFundación de Investigación HM Hospitales, HM Hospitales, C/Oña 10, 28050, Madrid, Spain.
R MadurgaFundación de Investigación HM Hospitales, HM Hospitales, C/Oña 10, 28050, Madrid, Spain.
G RackovFundación de Investigación HM Hospitales, HM Hospitales, C/Oña 10, 28050, Madrid, Spain.
I Palacín-AlianaFundación de Investigación HM Hospitales, HM Hospitales, C/Oña 10, 28050, Madrid, Spain.
R Núñez-TorresSpanish National Cancer Research Center (CNIO), 28029, Madrid, Spain.
A Asensi-PuigAtrys Health, Barcelona, Spain.
J Carrión-NavarroFundación de Investigación HM Hospitales, HM Hospitales, C/Oña 10, 28050, Madrid, Spain.
S Esteban-RubioFundación de Investigación HM Hospitales, HM Hospitales, C/Oña 10, 28050, Madrid, Spain.
H PeinadoSpanish National Cancer Research Center (CNIO), 28029, Madrid, Spain.
A González-NeiraSpanish National Cancer Research Center (CNIO), 28029, Madrid, Spain.
V González-RumayorAtrys Health, Barcelona, Spain.
C Belda-IniestaFundación de Investigación HM Hospitales, HM Hospitales, C/Oña 10, 28050, Madrid, Spain.
A Ayuso-SacidoFundación de Investigación HM Hospitales, HM Hospitales, C/Oña 10, 28050, Madrid, Spain. ayusosacido@gmail.com.ORCID 0000-0003-3919-5880

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundExtracellular vesicles (EVs) are small membrane-bound vesicles which play an important role in cell-to-cell communication. Their molecular cargo analysis is presented as a new source for biomarker detection, and it might provide an alternative to traditional solid biopsies. However, the most effective approach for EV isolation is not yet well established.

resultsHere, we study the efficiency of the most common EV isolation methods-ultracentrifugation, Polyethlyene glycol and two commercial kits, Exoquick

conclusionsOur findings present Polyethylene glycol precipitation as the most feasible and less cost-consuming EV isolation technique.

Indexed as

BiomarkersCell-Free Nucleic AcidsChemical PrecipitationExosomesExtracellular VesiclesHumansParticle SizePolyethylene GlycolsBiomarkersCell-Free Nucleic AcidsPolyethylene GlycolsExoQuick®Extracellular vesiclesLiquid biopsyPolyethylene glycolPureExo®Ultracentrifugation

Identifiers

PMID30871557
PMCPMC6419425

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.