Evidence map›Paper›PMID 30870780›Full record

ArticleiScience2019

Visco-Node-Pore Sensing: A Microfluidic Rheology Platform to Characterize Viscoelastic Properties of Epithelial Cells.

Junghyun Kim, Brian Li, Olivia J Scheideler, Youngbin Kim, Lydia L Sohn

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Review
  6. Article
  7. Review
  8. Article
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Junghyun KimDepartment of Mechanical Engineering, University of California at Berkeley, Berkeley, CA, USA.
Brian LiGraduate Program in Bioengineering, University of California, Berkeley, University of California, San Francisco, Berkeley, CA, USA.
Olivia J ScheidelerGraduate Program in Bioengineering, University of California, Berkeley, University of California, San Francisco, Berkeley, CA, USA.
Youngbin KimDepartment of Bioengineering, University of California at Berkeley, Berkeley, CA, USA.
Lydia L SohnDepartment of Mechanical Engineering, University of California at Berkeley, Berkeley, CA, USA; Graduate Program in Bioengineering, University of California, Berkeley, University of California, San Francisco, Berkeley, CA, USA. Electronic address: sohn@berkeley.edu.

Funding

Mechanical Phenotyping of Random Periaerolar Fine Needle Aspiration-Collected Cells for Early Breast Cancer DetectionR01EB024989 · NIBIB · UNIVERSITY OF CALIFORNIA BERKELEY · PI Mark A LaBarge, Lydia L Sohn · 2017 to 2026
$4.8M
A Label-Free, Point-of-Care Platform to Diagnose Acute Promyelocytic LeukemiaR01CA190843 · NCI · UNIVERSITY OF CALIFORNIA BERKELEY · PI FEUSNER, JAMES, SOHN, LYDIA L · 2015 to 2019
$1.6M
Isolating Circulating Tumor CellsR21CA182375 · NCI · UNIVERSITY OF CALIFORNIA BERKELEY · PI SOHN, LYDIA L · 2015 to 2017
$605k
NCI NIH HHS R01 CA190843NCI NIH HHS R21 CA182375NIBIB NIH HHS R01 EB024989
6 · The paper itself

Abstract

Viscoelastic properties of cells provide valuable information regarding biological or clinically relevant cellular characteristics. Here, we introduce a new, electronic-based, microfluidic platform-visco-node-pore sensing (visco-NPS)-which quantifies cellular viscoelastic properties under periodic deformation. We measure the storage (G') and loss (G″) moduli (i.e., elasticity and viscosity, respectively) of cells. By applying a wide range of deformation frequencies, our platform quantifies the frequency dependence of viscoelastic properties. G' and G″ measurements show that the viscoelastic properties of malignant breast epithelial cells (MCF-7) are distinctly different from those of non-malignant breast epithelial cells (MCF-10A). With its sensitivity, visco-NPS can dissect the individual contributions of different cytoskeletal components to whole-cell mechanical properties. Moreover, visco-NPS can quantify the mechanical transitions of cells as they traverse the cell cycle or are initiated into an epithelial-mesenchymal transition. Visco-NPS identifies viscoelastic characteristics of cell populations, providing a biophysical understanding of cellular behavior and a potential for clinical applications.

Indexed as

Analytical ChemistryBiomechanicsCellFluidics

Identifiers

PMID30870780
PMCPMC6416673

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.