ArticleNucleic acids research2019
Enzyme-free ligation of dimers and trimers to RNA primers.
Article in Nucleic acids research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Autonomous migration of a phosphate group along glycerol and inositol scaffolds driven by a phosphodiester reaction cycle.Nature chemistry · 2026Article
- Sequence patterns in RNA replication via the virtual circular genome mechanism.Nucleic acids research · 2026Article
- Evolved DNAzymes and Stable Activation Chemistry Enable High-Efficiency DNA Ligation.Chemistry (Weinheim an der Bergstrasse, Germany) · 2026Article
- Complex and Messy Prebiotic Chemistry: Obstacles and Opportunities for an RNA World.Life (Basel, Switzerland) · 2026Review
- Translation-Promoting Effects of RNA Template Overhangs in the Absence of Ribosomes.Angewandte Chemie (International ed. in English) · 2026Article
- From liquid-phase synthesis to chemical ligation: preparation of oligonucleotides and their backbone analogs in solution.Nucleic acids research · 2025Review
- Article
- A quantitative model of enzyme-free copying of RNA with dimers.Nucleic acids research · 2025Article
- The power of triplets.Nature reviews. Chemistry · 2025Article
- The XNA alphabet.Nucleic acids research · 2025Review
- Trinucleotide substrates under pH-freeze-thaw cycles enable open-ended exponential RNA replication by a polymerase ribozyme.Nature chemistry · 2025Article
- Selection-diversification interplay in oligonucleotide chemical evolution.Biophysics and physicobiology · 2025Article
- Overcoming nucleotide bias in the nonenzymatic copying of RNA templates.Nucleic acids research · 2024Article
- Template-based copying in chemically fuelled dynamic combinatorial libraries.Nature chemistry · 2024Article
- High-Fidelity RNA Copying via 2',3'-Cyclic Phosphate Ligation.Journal of the American Chemical Society · 2024Article
- Rolling Circles as a Means of Encoding Genes in the RNA World.Life (Basel, Switzerland) · 2022Article
- Enzyme-Free Copying of 12 Bases of RNA with Dinucleotides.Angewandte Chemie (International ed. in English) · 2022Article
- Thermodynamic and Kinetic Sequence Selection in Enzyme-Free Polymer Self-Assembly inside a Non-equilibrium RNA Reactor.Life (Basel, Switzerland) · 2022Article
- Nonenzymatic assembly of active chimeric ribozymes from aminoacylated RNA oligonucleotides.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Rolling circle RNA synthesis catalyzed by RNA.eLife · 2022Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The template-directed formation of phosphodiester bonds between two nucleic acid components is a pivotal process in biology. To induce such a reaction in the absence of enzymes is a challenge. This challenge has been met for the extension of a primer with mononucleotides, but the ligation of short oligonucleotides (dimers or trimers) has proven difficult. Here we report a method for ligating dimers and trimers of ribonucleotides using in situ activation in aqueous buffer. All 16 different dimers and two trimers were tested. Binding studies by NMR showed low millimolar dissociation constants for complexes between representative dimers and hairpins mimicking primer-template duplexes, confirming that a weak template effect is not the cause of the poor ligating properties of these short oligomers. Rather, cyclization was found to compete with ligation, with up to 90% of dimer being converted to the cyclic form during the course of an assay. This side reaction is strongly sequence dependent and more pronounced for dimers than for trimers. Under optimized reaction conditions, high yields were observed with strongly pairing purines at the 3'-terminus. These results show that short oligomers of ribonucleotides are competent reactants in enzyme-free copying.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.