ArticleEpidemiology and infection2019
A hospital qPCR-based survey of 10 gastrointestinal parasites in routine diagnostic screening, Marseille, France.
Article in Epidemiology and infection, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed.
- Occurrence and subtyping of the intestinal protozoanFood and waterborne parasitology · 2026Article
- Implementation of real-time PCR assays for diagnosing intestinal protozoa infections.Parasitology research · 2025Article
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- Current status of intestinal parasitosis and microsporidiosis in industrialized countries: Results from a prospective study in France and Luxembourg.PLoS neglected tropical diseases · 2024Article
- The Low Genetic Diversity of Dientamoeba fragilis Isolates in Southwest Turkey and Analysis of Clinical Findings.Acta parasitologica · 2024Article
- Evaluation of the Performance of the NovodiagPathogens (Basel, Switzerland) · 2023Article
- Prevalence of Blastocystis sp. in Morocco: Comparative assessment of three diagnostic methods and characterization of parasite forms in Jones' culture medium.Parasite (Paris, France) · 2023Article
- Rare Occurrence ofJournal of clinical medicine · 2022Article
- The NovodiagParasite (Paris, France) · 2022Article
- Diagnosis of Taenia solium infections based on "mail order" RNA-sequencing of single tapeworm egg isolates from stool samples.PLoS neglected tropical diseases · 2021Article
- Comparative Assessment of In-House Real-Time PCRs Targeting Enteric Disease-Associated Microsporidia in Human Stool Samples.Pathogens (Basel, Switzerland) · 2021Article
- Occurrence of Ten Protozoan Enteric Pathogens in Three Non-Human Primate Populations.Pathogens (Basel, Switzerland) · 2021Article
- Prevalence and Subtype Distribution ofMicroorganisms · 2020Article
- Natural Infection withmSphere · 2020Article
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6 authors.
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Abstract
There is a scarcity of recent epidemiological data on intestinal parasitic infections in France. We conducted a prospective study aimed at estimating the prevalence of 10 enteric parasites in Marseille, France, using real-time polymerase chain reaction (PCR)-based diagnosis. A total of 643 faeces from 488 patients referred to the Parasitology-Mycology Laboratory of the University Hospital of Marseille over a 6 months period were included. DNA was extracted using a semi-automated method. Parasites of interest were detected using singleplex quantitative PCRs (qPCRs). For positive samples, the Blastocystis subtype was determined by sequence analysis. During the study, the overall prevalence of enteric parasites was 17%. Blastocystis sp. was the most frequent species (10.5%), followed by Dientamoeba fragilis (2.3%) and Giardia intestinalis (2.3%). The prevalence of other parasites was <1% each. The ST3 Blastocystis subtype was predominant (43.6%) and the other subtypes identified were ST1, ST2, ST4 and ST6. This is the first time that a qPCR-based diagnosis has been used to survey the prevalence of 10 enteric parasites in a French University Hospital. This study confirms that fast, specific, sensitive and simultaneous detection in a single stool sample by qPCR clearly outperforms conventional microscopy-based diagnosis. Furthermore, qPCR is particularly well suited to surveying gastroenteritis agents.
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