ArticleEpidemiology and infection2019
Differential detection of Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii in faecal samples using nested multiplex PCR in west of Iran.
Article in Epidemiology and infection, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Assessment of water quality and microbial contamination in Santa Marta's major rivers using conventional methods and next-generation sequencing.Environmental science and pollution research international · 2026Article
- Unusual Findings of Human-Associated Four-NucleatedAnimals : an open access journal from MDPI · 2025Article
- Occult Amebiasis among Pediatric with Enterocolitis in National Referral Hospital in Indonesia.Infection & chemotherapy · 2024Article
- Molecular identification ofVeterinary world · 2024Article
- Development of nucleic acid lateral flow immunoassay for molecular detection of Entamoeba moshkovskii and Entamoeba dispar in stool samples.Scientific reports · 2024Article
- Article
- Metagenomic analysis reveals the relationship between intestinal protozoan parasites and the intestinal microecological balance in calves.Parasites & vectors · 2023Article
- Molecular-Genotyping Detection ofArchives of Razi Institute · 2023Article
- Prevalence of Human IntestinalPathogens (Basel, Switzerland) · 2022Review
- Molecular identification of Entamoeba histolytica, E. dispar, and E. moshkovskii in children with diarrhea from Maracaibo, VenezuelaBiomedica : revista del Instituto Nacional de Salud · 2021Article
- Molecular Identification and Prevalence ofPolish journal of microbiology · 2020Article
- Identification ofIranian journal of parasitologyArticle
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Authors and funding
4 authors.
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Abstract
This study aimed to determine the prevalence of Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii (collectively referred to as Entamoeba complex), using microscopic and molecular methods in Kurdistan Province, northwest of Iran. The relationship between positive Entamoeba species and clinical symptoms was also investigated. Eight positive Entamoeba complex, as well as four Entamoeba complex-like isolates, were detected by microscopic stool examination. DNA was extracted from all positive and from 55 randomly selected negative stool samples. PCR was performed using species-specific 18S rRNA primers for the Entamoeba complex. All positive PCR samples were sequenced. In total, 14 (1.01%) out of 1383 isolates, i.e. 12 microscopy-positive and Entamoeba complex-like isolates and two out of 55 microscopy-negative isolates, were identified via PCR and sequencing. Overall, 0.58% (8/1383) of the isolates were E. dispar, 0.14% (2/1383) E. histolytica, 0.07% (1/1383) E. moshkovskii and 0.22% (3/1383) were mixed of E. histolytica and E. dispar. Based on our findings, the prevalence of E. dispar is greater than that of E. histoltyica. On the other hand, a case of E. moshkovskii was reported for the first time in this region. It seems that some gastrointestinal symptoms may be attributed to Entamoeba species.
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