ArticleBiomicrofluidics2018
Label-free extraction of extracellular vesicles using centrifugal microfluidics.
Article in Biomicrofluidics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 49 citations in OpenAlex.
- Biogenesis and functional implications of extracellular vesicles in cancer metastasis.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2025Review
- Isolation methods of exosomes derived from dental stem cells.International journal of oral science · 2025Review
- Emerging Microfluidic Tools for Simultaneous Exosomes and Cargo Biosensing in Liquid Biopsy: New Integrated Miniaturized FFF-Assisted Approach for Colon Cancer Diagnosis.Sensors (Basel, Switzerland) · 2023Review
- Label-free approaches for extracellular vesicle detection.iScience · 2023Review
- Numerical Simulation of a Lab-on-Chip for Dielectrophoretic Separation of Circulating Tumor Cells.Micromachines · 2023Article
- Recent Developments in Inertial and Centrifugal Microfluidic Systems along with the Involved Forces for Cancer Cell Separation: A Review.Sensors (Basel, Switzerland) · 2023Review
- Updated Methods of Extracellular Vesicles Isolation.Advances in experimental medicine and biology · 2023Article
- Microfluidic Strategies for Extracellular Vesicle Isolation: Towards Clinical Applications.Biosensors · 2022Review
- Emerging micro-nanotechnologies for extracellular vesicles in immuno-oncology: from target specific isolations to immunomodulation.Lab on a chip · 2022Review
- Methodologies to Isolate and Purify Clinical Grade Extracellular Vesicles for Medical Applications.Cells · 2022Review
- Neural Network-Based Optimization of an Acousto Microfluidic System for Submicron Bioparticle Separation.Frontiers in bioengineering and biotechnology · 2022Article
- Micron-sized particle separation with standing surface acoustic wave-Experimental and numerical approaches.Ultrasonics sonochemistry · 2021Article
- Extracellular Vesicles as a Therapeutic Tool for Kidney Disease: Current Advances and Perspectives.International journal of molecular sciences · 2021Review
- Article
- Cancer cell enrichment on a centrifugal microfluidic platform using hydrodynamic and magnetophoretic techniques.Scientific reports · 2021Article
- Exploiting Microfluidics for Extracellular Vesicle Isolation and Characterization: Potential Use for Standardized Embryo Quality Assessment.Frontiers in veterinary science · 2020Review
- Microfluidic device for on-chip isolation and detection of circulating exosomes in blood of breast cancer patients.Biomicrofluidics · 2019Article
- DEP-on-a-Chip: Dielectrophoresis Applied to Microfluidic Platforms.Micromachines · 2019Review
- Challenges and opportunities in exosome research-Perspectives from biology, engineering, and cancer therapy.APL bioengineering · 2019Review
- The Use of Microfluidic Technology for Cancer Applications and Liquid Biopsy.Micromachines · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Extracellular vesicles (EVs) play an important role as active messengers in intercellular communication and distant microenvironment modeling. Increasingly, these EVs are recognized as important biomarkers for clinical diagnostics. However, current isolation methods of EVs are time-consuming and ineffective due to the high diffusive characteristics of nanoparticles coupled with fluid flow instability. Here, we develop a microfluidic CEntrifugal Nanoparticles Separation and Extraction (µCENSE) platform for the rapid and label-free isolation of microvesicles. By utilizing centrifugal microhydrodynamics, we subject the nanosuspensions between 100 nm and 1000 nm to a unique fluid flow resulting in a zonal separation into different outlets for easy post-processing. Our centrifugal platform utilizes a gentle and efficient size-based separation without the requirements of syringe pump and other accessories. Based on our results, we report a high separation efficiency of 90% and an extraction purity of 85% within a single platform. Importantly, we demonstrate high EV extraction using a table top centrifuge within a short duration of eight minutes. The simple processes and the small volume requirement further enhance the utility of the platform. With this platform, it serves as a potential for liquid biopsy extraction and point-of-care diagnostics.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.