Evidence map›Paper›PMID 30867549›Full record

ArticleScientific reports2019

Non-Invasive Monitoring of Functional State of Articular Cartilage Tissue with Label-Free Unsupervised Hyperspectral Imaging.

Saabah B Mahbub, Anna Guller, Jared M Campbell, Ayad G Anwer, Martin E Gosnell, Graham Vesey, Ewa M Goldys

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed, 2 pooled it
3.3field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 2 syntheses or guidelines pooled it, 33 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Saabah B MahbubARC Centre of Excellence for Nanoscale Biophotonics, Macquarie University, Sydney, NSW, Australia. s.mahbub@unsw.edu.au.
Anna GullerGraduate School of Biomedical Engineering, UNSW Australia, Sydney, NSW, 2052, Australia.
Jared M CampbellGraduate School of Biomedical Engineering, UNSW Australia, Sydney, NSW, 2052, Australia.
Ayad G AnwerGraduate School of Biomedical Engineering, UNSW Australia, Sydney, NSW, 2052, Australia.
Martin E GosnellARC Centre of Excellence for Nanoscale Biophotonics, Macquarie University, Sydney, NSW, Australia.
Graham VeseyRegeneus Pty Ltd, 25 Bridge Street, Pymble, NSW, 2073, Australia.
Ewa M GoldysGraduate School of Biomedical Engineering, UNSW Australia, Sydney, NSW, 2052, Australia. e.goldys@unsw.edu.au.
UNSW Sydney · AUMacquarie University · AUEngeneic (Australia) · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Damage and degradation of articular cartilage leads to severe pain and loss of mobility. The development of new therapies for cartilage regeneration for monitoring their effect requires further study of cartilage, ideally at a molecular level and in a minimally invasive way. Hyperspectral microscopy is a novel technology which utilises endogenous fluorophores to non-invasively assess the molecular composition of cells and tissue. In this study, we applied hyperspectral microscopy to healthy bovine articular cartilage and osteoarthritic human articular cartilage to investigate its capacity to generate informative molecular data and characterise disease state and treatment effects. We successfully demonstrated label-free fluorescence identification of collagen type I and II - isolated in cartilage here for the first time and the co-enzymes free NADH and FAD which together give the optical redox ratio that is an important measure of metabolic activity. The intracellular composition of chondrocytes was also examined. Differences were observed in the molecular ratios within the superficial and transitional zones of the articular cartilage which appeared to be influenced by disease state and treatment. These findings show that hyperspectral microscopy could be useful for investigating the molecular underpinnings of articular cartilage degradation and repair. As it is non-invasive and non-destructive, samples can be repeatedly assessed over time, enabling true time-course experiments with in-depth molecular data. Additionally, there is potential for the hyperspectral approach to be adapted for patient examination to allow the investigation of cartilage state. This could be of advantage for assessment and diagnosis as well as treatment monitoring.

Indexed as

AnimalsCartilage, ArticularCattleHumansIn Vitro TechniquesMaleMicroscopy, FluorescenceMiddle AgedOptical Imaging

Identifiers

PMID30867549
PMCPMC6416344
OpenAlexW2921758266

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.