ArticleBMC genomics2019
Reliable multiplex sequencing with rare index mis-assignment on DNB-based NGS platform.
Article in BMC genomics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
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- A tombus-like virus in patients with lower respiratory tract infection: an observational study based on meta-transcriptomic sequencing.Emerging microbes & infections · 2025Observational
- Gravitropic Gene Expression Divergence Associated With Adaptation to Contrasting Environments in an Australian Wildflower.Molecular ecology · 2025Article
- A targeted next-generation sequencing panel for identification of clinically relevant mutation profiles in solid tumours.Scientific reports · 2025Article
- DGCR8 haploinsufficiency leads to primate-specific RNA dysregulation and pluripotency defects.Nucleic acids research · 2025Article
- Article
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- Beyond the base pairs: comparative genome-wide DNA methylation profiling across sequencing technologies.Briefings in bioinformatics · 2024Article
- Epidemiology and Azole Resistance of Clinical Isolates ofInfection and drug resistance · 2024Article
- A comprehensive assessment of exome capture methods for RNA sequencing of formalin-fixed and paraffin-embedded samples.BMC genomics · 2023Review
- Enhanced specificity of Bacillus metataxonomics using a tuf-targeted amplicon sequencing approach.ISME communications · 2023Article
- Cross-platform comparisons for targeted bisulfite sequencing of MGISEQ-2000 and NovaSeq6000.Clinical epigenetics · 2023Article
- The Application of Metagenomics to Study Microbial Communities and Develop Desirable Traits in Fermented Foods.Foods (Basel, Switzerland) · 2022Review
- Sequencing introduced false positive rare taxa lead to biased microbial community diversity, assembly, and interaction interpretation in amplicon studies.Environmental microbiome · 2022Article
- Trafficked Malayan pangolins contain viral pathogens of humans.Nature microbiology · 2022Article
- Side-by-Side Comparison of Post-Entry Quarantine and High Throughput Sequencing Methods for Virus and Viroid Diagnosis.Biology · 2022Article
- Bioinformatics resources for SARS-CoV-2 discovery and surveillance.Briefings in bioinformatics · 2021Article
- Highly comparable metabarcoding results from MGI-Tech and Illumina sequencing platforms.PeerJ · 2021Article
Corrections and comments
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Authors and funding
27 authors.
Funding
Abstract
backgroundMassively-parallel-sequencing, coupled with sample multiplexing, has made genetic tests broadly affordable. However, intractable index mis-assignments (commonly exceeds 1%) were repeatedly reported on some widely used sequencing platforms.
resultsHere, we investigated this quality issue on BGI sequencers using three library preparation methods: whole genome sequencing (WGS) with PCR, PCR-free WGS, and two-step targeted PCR. BGI's sequencers utilize a unique DNA nanoball (DNB) technology which uses rolling circle replication for DNA-nanoball preparation; this linear amplification is PCR free and can avoid error accumulation. We demonstrated that single index mis-assignment from free indexed oligos occurs at a rate of one in 36 million reads, suggesting virtually no index hopping during DNB creation and arraying. Furthermore, the DNB-based NGS libraries have achieved an unprecedentedly low sample-to-sample mis-assignment rate of 0.0001 to 0.0004% under recommended procedures.
conclusionsSingle indexing with DNB technology provides a simple but effective method for sensitive genetic assays with large sample numbers.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.